Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds

Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds

The effect of solvent polarity on extraction yield and antioxidant properties of phytochemical compounds in bean seeds was studied. Seed flour of three varieties of bean was extracted in a series of organic solvents with increasing polarity (n-hexane, petroleum ether, chloroform, ethyl acetate, etha...

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Bibliographic Details
Published in:Brazilian Journal of Pharmaceutical Sciences Vol. 56
Main Authors: Nawaz, Haq, Shad, Muhammad Aslam, Rehman, Najiha, Andaleeb, Hina, Ullah, Najeeb
Format: Journal Article
Language:English
Published: Sao Paulo Universidade de Sao Paulo Faculdade de Ciencias 01-01-2020
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Universidade de São Paulo
Subjects:
Acids
Antioxidants
Beans
Beans seeds/antioxidant properties
Carotenoids
Ethanol
Flavonoids
Free radicals
Laboratories
PHARMACOLOGY & PHARMACY
Phaseolus vulgaris/phytochemical composition
Phytochemicals
Regression analysis
Seeds
Solvent polarity
Solvents
Variance analysis
Solvent polarity
Phaseolus vulgaris/phytochemical composition
Beans seeds/antioxidant properties
Regression analysis
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Summary:The effect of solvent polarity on extraction yield and antioxidant properties of phytochemical compounds in bean seeds was studied. Seed flour of three varieties of bean was extracted in a series of organic solvents with increasing polarity (n-hexane, petroleum ether, chloroform, ethyl acetate, ethanol, acetone and water). Preliminary screening of phytochemicals showed the presence of tannins, flavonoids, cardiac glycosides, anthocyanins, terpenoids, carotenoids, ascorbic acid and reducing compounds in all extracts. One way analysis of variance (ANOVA) of results showed that extraction yield, phytochemical content and antioxidant properties were significantly influenced (p<0.05) by the polarity of extracting solvents. The regression analysis of data showed polarity-dependent second order polynomial variations in the extraction yield, phytochemical contents, antioxidant activity, reducing properties and free radical scavenging activity of each variety. Extraction in highly polar solvents resulted in high extract yield but low phenolic and flavonoid content as compared to non-polar ones. The polarity-dependent increase in total antioxidant activity and reducing properties indicates the extraction of strong antioxidant compounds in polar solvents. The study suggests the use of a combination of polar and nonpolar solvents to increase the extraction efficiency of phytochemicals with good antioxidant quality from the bean and other legume seeds.
ISSN:2175-9790
1984-8250
2175-9790
DOI:10.1590/s2175-97902019000417129