Determination of Hepatoprotective Effects of Vanillin Against Lipopolysaccharide-induced Acute Liver Injury In-Vivo Mice Model
Background: Vanillin, a phenolic aldehyde extracted from Vanilla planifolia pods, has various biological activities, however, little is known about its hepatoprotective role. In this study, we developed an acute liver injury murine model using lipopolysaccharides (LPS) and elucidated the hepatoprot...
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| Published in: | Pakistan Journal of Medicine and Dentistry Vol. 13; no. 3 |
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| Main Authors: | , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
ziauddin University
01-08-2024
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| Online Access: | Get full text |
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| Summary: | Background: Vanillin, a phenolic aldehyde extracted from Vanilla planifolia pods, has various biological activities, however, little is known about its hepatoprotective role. In this study, we developed an acute liver injury murine model using lipopolysaccharides (LPS) and elucidated the hepatoprotective activity of vanillin. Methods: A pre-clinical study using a random sampling technique was conducted at Ziauddin University MDRL-1 and 2 labs, between March-December 2023. The inclusion criteria included 36 healthy male BALB/c. Six groups (n=6) of male BALB/c mice (25-30g) were used: Normal, LPS, Treatment groups (two different concentrations of Vanillin were intraperitoneally [I.P.] given to mice), toxicity, and positive control group respectively. The animals were pre-treated for four days and a single dose of LPS (2mg/kg) was I.P. administered one day before euthanizing animals. Using SPSS v.24, one-way ANOVA was performed followed by Tukey and Bonferroni’s post hoc for results comparison between different groups with p<0.05 significant. Results: The results indicated that vanillin had a markedly protective effect on acute liver injury induced by LPS in mice. Histopathological analysis showed that vanillin administration minimized liver injury levels. Also, the administration of vanillin inhibited the pro-inflammatory cytokine IL-6 growth in liver tissue. A significant elevation of liver enzymes in LPS-treated mice (2 mg/kg); AST and ALT activities were 360.63 ±14.56 U/L (p<0.0001) and 188.50 ±10.55 U/L versus 98.73 ±4.4 U/L and 36.53 ±3.56 U/L in the control group were seen. Conclusion: Our findings suggest that vanillin might be a promising candidate for LPS-induced acute liver injury via its regulating effects on inflammation in the hepatic cells. Keywords: Lipopolysaccharide, Vanillin, Hepatoprotective, Acute Liver Injury, Inflammation. |
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| ISSN: | 2313-7371 2308-2593 |
| DOI: | 10.36283/PJMD13-3/008 |