Improved dark fermentation of cane molasses in mesophilic and thermophilic anaerobic fluidized bed reactors by selecting operational conditions
Summary Sugarcane molasses is a carbohydrate‐rich carbon source with potential to develop the biorefinery model in the sugarcane industry. Two of the most well‐known renewable products from the dark fermentation of cane molasses are succinic acid and biohydrogen, with both having valuable applicatio...
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| Published in: | International journal of energy research Vol. 44; no. 13; pp. 10442 - 10452 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Chichester, UK
John Wiley & Sons, Inc
25-10-2020
Hindawi Limited |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Summary
Sugarcane molasses is a carbohydrate‐rich carbon source with potential to develop the biorefinery model in the sugarcane industry. Two of the most well‐known renewable products from the dark fermentation of cane molasses are succinic acid and biohydrogen, with both having valuable applications in different industries. However, the continuous dark fermentation of cane molasses is a process regulated by many operational conditions, like reactor temperature and hydraulic retention time. It is important to control these conditions to shift metabolic pathways in order to increase production rates and yields. Thus, this research evaluates the response of mesophilic and thermophilic dark fermentation of cane molasses by mixed culture on hydrogen and succinic acid generation in fluidized bed reactors by changing the hydraulic retention time (8, 6, 4, 2, and 1 hour). In the thermophilic reactor (55°C), higher hydraulic retention times (from 8 to 4 hours) favored the succinate production (molar fractions between 75.0% and 81.4%). Similarly, in the mesophilic reactor (30°C), the molar fractions of succinate were 45.5% at the hydraulic retention time of 8 hours and 74.7% at 6 hours. The reduction of the hydraulic retention time to 1 hour decreased the succinic acid concentration to undetectable values and increased the hydrogen yield in both reactors. The lower hydraulic retention time also enhanced the hydrogen productivity to 171.1 mL/Lbed·h in the mesophilic reactor and to 303.4 mL/Lbed·h in the thermophilic reactor. These values were coincident with the predominant production of butyric and acetic acids. All of these points to the fact that the hydraulic retention time is an efficient parameter to control and select the production of hydrogen or succinic acid by mixed cultures under different temperatures.
To our knowledge, this is the first study to compare mesophilic and thermophilic conditions for the continuous dark fermentation of sugarcane molasses in anaerobic fluidized bed reactors using different mixed cultures. Based on the results observed, the variation of hydraulic retention time can select the microbial populations and the main metabolic pathways between succinic acid and hydrogen production routes in high‐rate mesophilic and thermophilic reactors. This enables the adoption of precise operational conditions depending on the targeted product. |
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| Bibliography: | Funding information Conselho Nacional de Desenvolvimento Científico e Tecnológico ‐ Brasil (CNPq); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ‐ Brasil (CAPES), Grant/Award Number: Finance Code 001; Fundação de Amparo à Pesquisa do Estado de São Paulo ‐ Brasil (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico; National Council for Scientific and Technological Development; Coordenação de Aperfeiçoamento de Pessoal de nível superior; Coordination for the Improvement of Higher Education Personnel; Fundação de Amparo à Pesquisa do Estado de São Paulo; Foundation for Research Support of the State of São Paulo |
| ISSN: | 0363-907X 1099-114X |
| DOI: | 10.1002/er.5673 |