Search Results - "Rees, Johanna S."

In Vivo Analysis of Proteomes and Interactomes Using Parallel Affinity Capture (iPAC) Coupled to Mass Spectrometry by Rees, Johanna S., Lowe, Nick, Armean, Irina M., Roote, John, Johnson, Glynnis, Drummond, Emma, Spriggs, Helen, Ryder, Edward, Russell, Steven, Johnston, Daniel St, Lilley, Kathryn S.

“…Affinity purification coupled to mass spectrometry provides a reliable method for identifying proteins and their binding partners. In this study we have used…”
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Drosophila Ionotropic Receptor 25a mediates circadian clock resetting by temperature by Chen, Chenghao, Buhl, Edgar, Xu, Min, Croset, Vincent, Rees, Johanna S., Lilley, Kathryn S., Benton, Richard, Hodge, James J. L., Stanewsky, Ralf

“…A Drosophila chemosensory receptor, expressed in leg sensory neurons, is necessary for behavioural and molecular synchronization of the fly’s circadian clock…”
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Protein Neighbors and Proximity Proteomics by Rees, Johanna S., Li, Xue-Wen, Perrett, Sarah, Lilley, Kathryn S., Jackson, Antony P.

“…Within cells, proteins can co-assemble into functionally integrated and spatially restricted multicomponent complexes. Often, the affinities between individual…”
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The chicken B-cell line DT40 proteome, beadome and interactomes by Rees, Johanna S., Lilley, Kathryn S., Jackson, Antony P.

“…In developing a new quantitative AP-MS method for exploring interactomes in the chicken B-cell line DT40, we also surveyed the most abundant proteins in this…”
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The β3-subunit modulates the effect of venom peptides ProTx-II and OD1 on Na V 1.7 gating by Salvage, Samantha C, Rahman, Taufiq, Eagles, David A, Rees, Johanna S, King, Glenn F, Huang, Christopher L-H, Jackson, Antony P

“…The voltage-gated sodium channel Na 1.7 is involved in various pain phenotypes and is physiologically regulated by the Na -β3-subunit. Venom toxins ProTx-II…”
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New Insights into the DT40 B Cell Receptor Cluster Using a Proteomic Proximity Labeling Assay by Li, Xue-Wen, Rees, Johanna S., Xue, Peng, Zhang, Hong, Hamaia, Samir W., Sanderson, Bailey, Funk, Phillip E., Farndale, Richard W., Lilley, Kathryn S., Perrett, Sarah, Jackson, Antony P.

“…In the vertebrate immune system, each B-lymphocyte expresses a surface IgM-class B cell receptor (BCR). When cross-linked by antigen or anti-IgM antibody, the…”
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The β3‐subunit modulates the effect of venom peptides ProTx‐II and OD1 on NaV1.7 gating by Salvage, Samantha C., Rahman, Taufiq, Eagles, David A., Rees, Johanna S., King, Glenn F., Huang, Christopher L‐H., Jackson, Antony P.

“…The voltage‐gated sodium channel NaV1.7 is involved in various pain phenotypes and is physiologically regulated by the NaV‐β3‐subunit. Venom toxins ProTx‐II…”
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Proteomic analysis in primary T cells reveals IL-7 alters T cell receptor thresholding via CYTIP/cytohesin/LFA-1 localisation and activation by Queiroz, Rayner M L, Piper, Siân C, Rees, Johanna S, Strickson, Sam, Briend, Emmanuel, Low, Choon Pei, Ferguson, G John, Lilley, Kathryn S, Jackson, Antony P, Finch, Donna K

“…The ability of the cellular immune system to discriminate self from foreign antigens depends on the appropriate calibration of the T cell receptor (TCR)…”
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Supramolecular clustering of the cardiac sodium channel Nav1.5 in HEK293F cells, with and without the auxiliary β3‐subunit by Salvage, Samantha C., Rees, Johanna S., McStea, Alexandra, Hirsch, Michael, Wang, Lin, Tynan, Christopher J., Reed, Matthew W., Irons, Jennifer R., Butler, Richard, Thompson, Andrew J., Martin-Fernandez, Marisa L., Huang, Christopher L.‐H., Jackson, Antony P.

“…Voltage‐gated sodium channels comprise an ion‐selective α‐subunit and one or more associated β‐subunits. The β3‐subunit (encoded by the SCN3B gene) is an…”
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Identification of the cis‑molecular neighbours of the immune checkpoint protein B7‑H4 in the breast cancer cell‑line SK‑BR‑3 by proteomic proximity labelling by Rees, Johanna S, Cheung, Lawrence C C, Hamaia, Samir W, Davies, Gareth, Sandercock, Alan, Lilley, Kathryn S, Tigue, Natalie, Jackson, Antony P

“…The immune checkpoint protein B7‑H4 plays an important role in the positive as well as the negative regulation of immune T‑cell responses. When expressed on…”
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SILAC-iPAC: A quantitative method for distinguishing genuine from non-specific components of protein complexes by parallel affinity capture by Rees, Johanna S., Lilley, Kathryn S., Jackson, Antony P.

“…Pull-down assays can identify members of protein complexes but suffer from co-isolation of contaminants. The problem is particularly acute when the…”
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Peritrophic membrane contribution to Bt Cry δ-endotoxin susceptibility in Lepidoptera and the effect of Calcofluor by Rees, Johanna S., Jarrett, Paul, Ellar, David J.

“…In an effort to determine a reason for differing susceptibilities to Bacillus thuringiensis (Bt) Cry δ-endotoxins amongst lepidopteran species, the peritrophic…”
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Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library by Lowe, Nick, Rees, Johanna S, Roote, John, Ryder, Ed, Armean, Irina M, Johnson, Glynnis, Drummond, Emma, Spriggs, Helen, Drummond, Jenny, Magbanua, Jose P, Naylor, Huw, Sanson, Bénédicte, Bastock, Rebecca, Huelsmann, Sven, Trovisco, Vitor, Landgraf, Matthias, Knowles-Barley, Seymour, Armstrong, J Douglas, White-Cooper, Helen, Hansen, Celia, Phillips, Roger G, Lilley, Kathryn S, Russell, Steven, St Johnston, Daniel

“…Although we now have a wealth of information on the transcription patterns of all the genes in the Drosophila genome, much less is known about the properties…”
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The β3-subunit modulates the effect of venom peptides ProTx-II and OD1 on NaV 1.7 gating by Salvage, Samantha C, Rahman, Taufiq, Eagles, David A, Rees, Johanna S, King, Glenn F, Huang, Christopher L-H, Jackson, Antony P

“…The voltage-gated sodium channel NaV 1.7 is involved in various pain phenotypes and is physiologically regulated by the NaV -β3-subunit. Venom toxins ProTx-II…”
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New Insights into the DT40 B Cell Receptor Cluster Using a Proteomic Proximity Labeling Assay<inline-graphic xlink:href="sbox.jpg"/> by Li, Xue-Wen, Rees, Johanna S, Xue, Peng, Zhang, Hong, Hamaia, Samir W, Sanderson, Bailey, Funk, Phillip E, Farndale, Richard W, Lilley, Kathryn S, Perrett, Sarah, Jackson, Antony P

“…Background: B cell receptor (BCR) clusters modulate BCR signaling in B-lymphocytes. Results: We used a quantitative proteomic proximity assay to analyze the…”
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