Quantitative serum proteome analysis using tandem mass tags in dogs with epilepsy

Quantitative serum proteome analysis using tandem mass tags in dogs with epilepsy

This study included four groups of dogs (group A: healthy controls, group B: idiopathic epilepsy receiving antiepileptic medication (AEM), group C: idiopathic epilepsy without AEM, group D: structural epilepsy). Comparative quantitative proteomic analysis of serum samples among the groups was the ma...

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Bibliographic Details
Published in:Journal of proteomics Vol. 290; p. 105034
Main Authors: Baka, Rania D., Kuleš, Josipa, Beletić, Anđelo, Farkaš, Vladimir, RešetarMaslov, Dina, Ljubić, Blanka Beer, Rubić, Ivana, Mrljak, Vladimir, McLaughlin, Marκ, Eckersall, David, Polizopoulou, Zoe
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 06-01-2024
Subjects:
Animals
Dog
Dogs
Epilepsy - veterinary
Haptoglobin binding assay
Idiopathic epilepsy
Proteome - metabolism
Proteomics
Serum
Specific ELISA assay
Tandem Mass Spectrometry
TMT-based proteomics
Haptoglobin binding assay
Serum
TMT-based proteomics
Specific ELISA assay
Dog
Idiopathic epilepsy
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Summary:This study included four groups of dogs (group A: healthy controls, group B: idiopathic epilepsy receiving antiepileptic medication (AEM), group C: idiopathic epilepsy without AEM, group D: structural epilepsy). Comparative quantitative proteomic analysis of serum samples among the groups was the main target of the study. Samples were analyzed by a quantitative Tandem-Mass-Tags approach on the Q-Exactive-Plus Hybrid Quadrupole-Orbitrap mass-spectrometer. Identification and relative quantification were performed in Proteome Discoverer. Data were analyzed using R. Gene ontology terms were analyzed based on Canis lupus familiaris database. Data are available via ProteomeXchange with identifier PXD041129. Eighty-one proteins with different relative adundance were identified in the four groups and 25 were master proteins (p < 0.05). Clusterin (CLU), and apolipoprotein A1 (APOA1) had higher abundance in the three groups of dogs (groups B, C, D) compared to controls. Amine oxidase (AOC3) was higher in abundance in group B compared to groups C and D, and lower in group A. Adiponectin (ADIPOQ) had higher abundance in groups C compared to group A. ADIPOQ and fibronectin (FN1) had higher abundance in group B compared to group C and D. Peroxidase activity assay was used to quantify HP abundance change, validating and correlating with proteomic analysis (r = 0.8796). The proteomic analysis of serum samples from epileptic dogs indicated potential markers of epilepsy (CLU), proteins that may contribute to nerve tissue regeneration (APOA1), and contributing factors to epileptogenesis (AOC3). AEM could alter extracellular matrix proteins (FN1). Illness (epilepsy) severity could influence ADIPOQ abundance. [Display omitted] •Serum proteome profile significantly differed between epileptic and healthy dogs.•Haptoglobin abundance changes correlated with peroxidase activity assay results.•Serum clusterin abundance changes may indicate its prognostic role in epilepsy.•Serum apolipoprotein A1 and amine oxidase changes may contribute to epileptogenesis.•Serum fibronectin abundance change may be altered by antiepileptic medication.
ISSN:1874-3919
1876-7737
DOI:10.1016/j.jprot.2023.105034