The production of monoclonal antibodies to human chorionic gonadotrophin and its subunits

The production of monoclonal antibodies to human chorionic gonadotrophin and its subunits

The difficulties encountered in producing highly specific antisera to human chorionic gonadotrophin (hCG) were overcome by the use of hybridoma technology. A panel of monoclonal antibodies directed toward hCG and its subunits was produced. Of the four antibodies which were fully characterized, one r...

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Bibliographic Details
Published in:Journal of endocrinology Vol. 98; no. 3; p. 323
Main Authors: Stuart, M C, Underwood, P A, Harman, D F, Payne, K L, Rathjen, D A, Razziudin, S, Von Sturmer, S R, Vines, K
Format: Journal Article
Language:English
Published: England 01-09-1983
Subjects:
Animals
Antibodies, Monoclonal - biosynthesis
Antibody Specificity
Chorionic Gonadotropin - immunology
Chorionic Gonadotropin, beta Subunit, Human
Cross Reactions
Female
Glycoprotein Hormones, alpha Subunit
Hybridomas - immunology
Immunoglobulin G - biosynthesis
Immunologic Techniques
Mice
Mice, Inbred BALB C
Peptide Fragments - immunology
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Summary:The difficulties encountered in producing highly specific antisera to human chorionic gonadotrophin (hCG) were overcome by the use of hybridoma technology. A panel of monoclonal antibodies directed toward hCG and its subunits was produced. Of the four antibodies which were fully characterized, one recognized the intact hCG molecule only, a second recognized only the free beta-subunit, a third recognized only the free alpha-subunit and the fourth bound to the beta-subunit of hCG both when it was in the free form and when it was associated with the alpha-subunit forming the intact hCG molecule. There was no significant cross-reaction of any of these antibodies with the pituitary glycoprotein hormones. The four antibodies had high binding affinities which should permit their use in immunoassays for measurement of circulating levels of hCG and its subunits.
ISSN:0022-0795
DOI:10.1677/joe.0.0980323