Search Results - "Raymond, J.A."

Functional evaluation of candidate ice structuring proteins using cell-free expression systems by Brödel, A.K., Raymond, J.A., Duman, J.G., Bier, F.F., Kubick, S.

“…► We describe cell-free expression methods for three ISPs that differ widely in structure and glycosylation status. ► We use both prokaryotic and eukaryotic…”
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An assay for acidic peptide substrates of protein kinases by Budde, R J, McMurray, J S, Tinker, D A

“…The assay of acidic peptides as substrates for protein kinases has not been as easy to perform as testing basic peptides or polypeptides. We have developed a…”
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Studies on the dark/light regulation of maize leaf pyruvate, orthophosphate dikinase by reversible phosphorylation by Budde, R J, Holbrook, G P, Chollet, R

“…In maize leaves, pyruvate, orthophosphate dikinase (PPDK) is deactivated in the dark and reactivated in the light. Studies in vitro using purified PPDK and a…”
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Requirement for an Additional Divalent Metal Cation To Activate Protein Tyrosine Kinases by Sun, Gongqin, Budde, Raymond J. A

“…In addition to the magnesium ion needed to form the true phosphate-donating substrate (ATP−Mg complex), we have determined that at least one additional Mg2+…”
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Use of synthetic peptides and copolymers to study the substrate specificity and inhibition of the protein tyrosine kinase pp60c-src by Budde, Raymond J.A., Obeyesekere, Nihal U., Ke, Shi, McMurray, John S.

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Autophosphorylation of Src and Yes blocks their inactivation by Csk phosphorylation by GONGQIN SUN, SHARMA, A. K, BUDDE, R. J. A

“…Csk phosphorylates Src family protein tyrosine kinases on a tyrosine residue near their C-terminus and downregulates their activity. We previously observed…”
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Mutations in the N-Terminal Regulatory Region Reduce the Catalytic Activity of Csk, but Do Not Affect Its Recognition of Src by Sun, Gongqin, Budde, Raymond J.A.

“…In addition to the C-terminal catalytic domain, Csk is a protein tyrosine kinase that has an N-terminal regulatory region that contains SH3 and SH2 domains…”
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Substitution Studies of the Second Divalent Metal Cation Requirement of Protein Tyrosine Kinase CSK by Sun, Gongqin, Budde, Raymond J. A

“…In addition to a magnesium ion needed to form the ATP−Mg complex, we have previously determined that at least one more free Mg2+ ion is essential for the…”
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Effect of Autophosphorylation on the Catalytic and Regulatory Properties of Protein Tyrosine Kinase Src by Sun, Gongqin, Ramdas, Latha, Wang, Wei, Vinci, Jennifer, McMurray, John, Budde, Raymond J.A.

“…The function of autophosphorylation in Src family protein tyrosine kinases is not fully understood. In this paper we compared the catalytic and ligand-binding…”
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Affinity Purification of Csk Protein Tyrosine Kinase Based on Its Catalytic Requirement for Divalent Metal Cations by Sun, Gongqin, Budde, Raymond J.A.

“…Protein tyrosine kinase Csk requires two Mg2+ ions for activity: one magnesium is part of the ATP–Mg complex, and the second free Mg2+ ion is required as an…”
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Csk Phosphorylation and Inactivationin Vitroby the cAMP-Dependent Protein Kinase by Sun, Gongqin, Ke, Shi, Budde, Raymond J.A.

“…Csk is a protein tyrosine kinase that phosphorylates other protein tyrosine kinases of the Src family and down-regulates their activities. It is not known how…”
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A Modified pGEX Expression System That Eliminates Degradation Products and Thrombin from the Recombinant Protein by Sun, Gongqin, Budde, Raymond J.A.

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Expression, Purification, and Initial Characterization of Human Yes Protein Tyrosine Kinase from a Bacterial Expression System by Sun, Gongqin, Budde, Raymond J.A.

“…Protein tyrosine kinase Yes is a cellular homolog of v-Yes, the oncogenic protein product of avian sarcoma virus Y73. Yes is a member of the Src family and its…”
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Csk phosphorylation and inactivation in vitro by the cAMP-dependent protein kinase by Sun, G, Ke, S, Budde, R J

“…Csk is a protein tyrosine kinase that phosphorylates other protein tyrosine kinases of the Src family and down-regulates their activities. It is not known how…”
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Tight-binding inhibitory sequences against pp60(c-src) identified using a random 15-amino-acid peptide library by Nishi, T, Budde, R J, McMurray, J S, Obeyesekere, N U, Safdar, N, Levin, V A, Saya, H

“…A bacteriophage peptide library containing a random 15-amino-acid insert was screened for identification of peptide sequence(s) that bind pp60(c-src)…”
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Benzodiazepine Compounds as Inhibitors of the Src Protein Tyrosine Kinase: Screening of a Combinatorial Library of 1,4-Benzodiazepines by Ramdas, Latha, Bunnin, Barry A., Plunkett, Matthew J., Sun, Gongqin, Ellman, Jonathan, Gallick, Gary, Budde, Raymond J.A.

“…We screened 1680 spatially separated compounds of a diverse combinatorial library of 1,4-benzodiazepines for their ability to inhibit the kinase activity of…”
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Cyclic peptides incorporating 4-carboxyphenylalanine and phosphotyrosine are potent inhibitors of pp60(c-)(src) by Wang, W, Ramdas, L, Sun, G, Ke, S, Obeyesekere, N U, Budde, R J, McMurray, J S

“…The protein tyrosine kinase, pp60(c-)(src), is involved in cellular signaling and is activated during mitosis and in various tumors. We have been employing…”
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An Automated Liquid-Phase Assay for Quantitation of Protein Tyrosine Kinase Activity by Ramdas, Latha, Budde, Raymond J.A.

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Cloning, expression, purification and characterization of the alternate splice Src variants for drug discovery by Budde, Raymond J.A., Ramdas, Latha, Sun, Gongqin

“…Protein tyrosine kinases (PTKs) are key members of intra- and extra-cellular signaling pathways. Aberrant signaling pathways are responsible for many human…”
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Pea leaf mitochondrial pyruvate dehydrogenase complex is inactivated in vivo in a light-dependent manner by Budde, R.J.A. (University of Missouri, Columbia, MO), Randall, D.D

“…We examined the effect of light on the activity of the mitochondrial pyruvate dehydrogenase complex (mt-PDC) by using intact green pea (Pisum sativum)…”
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