Preparation of Factor IX Deficient Human Plasma by Immunoaffinity Chromatography Using a Monoclonal Antibody

A murine hybridoma clone is described that grows continuously in culture and produces a monoclonal antibody we have called Royal Free Monoclonal Antibody to factor IX No. 1 (RFF-IX/1). This has high affinity for a coagulation site on factor IX. RFF-IX/1 immobilised on sepharose can be used to deplet...

Full description

Saved in:

Bibliographic Details
Published in:	Blood Vol. 59; no. 3; pp. 664 - 670
Main Authors:	Goodall, A.H., Kemble, G., O'Brien, D.P., Rawiings, E., Rotblat, F., Russell, G.C., Janossy, G., Tuddenham, E.G.D.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-03-1982
Subjects:	Animals Antibodies, Monoclonal - biosynthesis Chromatography, Affinity Factor IX - antagonists & inhibitors Factor IX - immunology Factor IX - isolation & purification Hemophilia B - blood Hemophilia B - diagnosis Humans Indicators and Reagents Mice Mice, Inbred BALB C
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A murine hybridoma clone is described that grows continuously in culture and produces a monoclonal antibody we have called Royal Free Monoclonal Antibody to factor IX No. 1 (RFF-IX/1). This has high affinity for a coagulation site on factor IX. RFF-IX/1 immobilised on sepharose can be used to deplete factor IX from normal human plasma. This immunoaffinity depleted plasma is indistinguishable from severe Christmas disease plasma and can be used as the substrate in a one stage coagulation assay for factor IX. The affinity column has high capacity and can be regenerated so that large scale production from normal plasma of factor IX deficient plasma as a diagnostic reagent is now feasible.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0006-4971 1528-0020
DOI:	10.1182/blood.V59.3.664.664