cDNA sequences and organization of IgM heavy chain genes in two holostean fish

Immunoglobulin M heavy chain (μ) sequences of two holostean fish, the bowfin, Amia calva, and the longnose gar, Lepisosteus osseus, were amplified from spleen mRNA by RACE-PCR, cloned, and sequenced. Each μ chain showed the conserved four constant domain structure typical of a secreted μ chain. Sout...

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Bibliographic Details
Published in:	Developmental and comparative immunology Vol. 19; no. 2; pp. 153 - 164
Main Authors:	Wilson, Melanie R., Ravenstein, Eric van, Miller, Norman W., Clem, L.William, Middleton, Darlene L., Warr, Gregory W.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Ltd 01-03-1995
Subjects:	Amino Acid Sequence Animals Base Sequence Bowfin cDNA sequence DNA, Complementary - isolation & purification Fishes - genetics Gar Holostean fish Immunoglobulin Constant Regions - genetics Immunoglobulin gene organization Immunoglobulin Heavy Chains - genetics Immunoglobulin M - genetics Immunoglobulin mu-Chains - genetics Molecular Sequence Data Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid cDNA sequence Gar Bowfin Immunoglobulin gene organization Holostean fish
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Summary:	Immunoglobulin M heavy chain (μ) sequences of two holostean fish, the bowfin, Amia calva, and the longnose gar, Lepisosteus osseus, were amplified from spleen mRNA by RACE-PCR, cloned, and sequenced. Each μ chain showed the conserved four constant domain structure typical of a secreted μ chain. Southern blot analyses with specific heavy chain variable (V H) and constant (C H) region probes suggest that both fish possess an IgH locus that resembles that of the teleosts, amphibians, and mammals in its organization. The overall sequence similarity of gar and bowfin μ chains was 60% and 48% at the nucleotide and amino acid levels, respectively, while similarity to the μ chains of teleosts and elasmobranchs was lower. The bowfin μ chain possesses a distinctive proline-rich sequence at the Cμ1/Cμ2 boundary; a shorter proline-rich sequence is present at this position in the gar μ chain. Both gar and bowfin show, in their Cμ4 sequences, motifs that could serve as cryptic splice donor sites for the production of mRNA encoding the membrane-bound form of the μ chains, and the bowfin also shows a potential cryptic splice donor site in the Cμ3 exon.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0145-305X 1879-0089
DOI:	10.1016/0145-305X(94)00063-L