Bioprospecting of lipolytic microorganisms obtained from industrial effluents

The lipases have ability to catalyze diverse reactions and are important in different biotechnological applications. The aim of this work was to isolate and characterize microorganisms that produce lipases, from different food industry effluents localized in Pelotas, RS/Brazil. Bacteria were identif...

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Published in:	Anais da Academia Brasileira de Ciências Vol. 88; no. 3 Suppl; pp. 1769 - 1779
Main Authors:	Peil, Greice H S, Kuss, Anelise V, Rave, Andrés F G, Villarreal, José P V, Hernandes, Yohana M L, Nascente, Patrícia S
Format:	Journal Article
Language:	English
Published:	Brazil Academia Brasileira de Ciências 2016
Subjects:	atividade enzimática Bacteria - enzymology Bacteria - isolation & purification Bioprospecting Brazil efluente Enterobacter aerogenes - enzymology Enterobacter aerogenes - isolation & purification enzima lipase Fungi - enzymology Fungi - isolation & purification Industrial Waste Lipase - biosynthesis microrganismo lipolítico MULTIDISCIPLINARY SCIENCES produção de lipase extracelular lipase enzyme enzima lipase effluent enzyme activity atividade enzimática extracellular lipase production produção de lipase extracelular lipolytic microorganism efluente microrganismo lipolítico
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Summary:	The lipases have ability to catalyze diverse reactions and are important in different biotechnological applications. The aim of this work was to isolate and characterize microorganisms that produce lipases, from different food industry effluents localized in Pelotas, RS/Brazil. Bacteria were identified using Gram stain and biochemical tests (Vitek 2(r)). Fungi were identified according to macro and micromorphology characteristics. The extracellular lipase production was evaluated using the Rhodamine B test and the enzymatic activity by titration. Twenty-one bacteria were isolated and identified as Klebsiella pneumoniae ssp. pneumoniae, Serratia marcescens, Enterobacter aerogenes, Raoultella ornithinolytica and Raoultella planticola. Were characterized isolated filamentous fungi by the following genera: Alternaria sp., Fusarium sp., Geotrichum sp., Gliocladium sp., Mucor sp., Paecilomyces sp. and Trichoderma sp. Extracellular lipase production was observed in 71.43% of the bacteria and 57.14% of the fungi. The bacterium that presented better promising enzymatic activity was E. aerogenes (1.54 U/ml) however between fungi there was not significant difference between the four isolates. This study indicated that microorganisms lipase producers are present in the industrial effluents, as well as these enzymes have potential of biodegradation of lipid compounds.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0001-3765 1678-2690 1678-2690
DOI:	10.1590/0001-3765201620150550