Marker assisted introgression of blast (Pi-2 and Pi-54) genes in to the genetic background of elite, bacterial blight resistant indica rice variety, Improved Samba Mahsuri

Marker assisted introgression of blast (Pi-2 and Pi-54) genes in to the genetic background of elite, bacterial blight resistant indica rice variety, Improved Samba Mahsuri

Improved Samba Mahsuri (ISM), a bacterial blight (BB) resistant (possessing Xa21, xa13 and xa5 ), high-yielding rice variety possessing fine-grain type has been recently released for cultivation and is becoming increasingly popular across many parts of India. However, the new variety is highly susce...

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Bibliographic Details
Published in:Euphytica Vol. 212; no. 2; pp. 331 - 342
Main Authors: Ratna Madhavi, K., Rambabu, R., Abhilash Kumar, V., Vijay Kumar, S., Aruna, J., Ramesh, S., Sundaram, R. M., Laha, G. S., Sheshu Madhav, M., Ravindra babu, V., Prasad, M. Srinivas
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01-11-2016
Springer
Springer Nature B.V
Subjects:
Bacteria
Biomedical and Life Sciences
Biotechnology
Cultivars
Disease resistance
Disease susceptibility
Disease transmission
Explosions
Genes
Genetic markers
Genetic research
Genotype & phenotype
Life Sciences
Medical screening
Plant diseases
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Rice
Selective breeding
Marker assisted introgression
Blast
Marker assisted selection
Rice
Bacterial blight
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Summary:Improved Samba Mahsuri (ISM), a bacterial blight (BB) resistant (possessing Xa21, xa13 and xa5 ), high-yielding rice variety possessing fine-grain type has been recently released for cultivation and is becoming increasingly popular across many parts of India. However, the new variety is highly susceptible to blast disease, which limits its spread. In the present study, we attempted to improve blast resistance of ISM by introgressing two major blast resistance genes ( Pi - 2 and Pi - 54 ) by marker-assisted backcross breeding through two separate sets of backcrosses, one involving transfer of Pi - 2 and another involving Pi - 54 . At each backcross generation, closely linked PCR-based markers were used to select plants possessing either Pi - 2 (first cross) or Pi - 54 (second cross) along with Xa21, xa13 and microsatellite markers polymorphic between donor and recurrent parents were used to select plants that have maximum contribution from the recurrent parent genome (background selection). Selected BC 2 F 1 plant possessing Pi - 2 was intercrossed with a selected BC 2 F 1 plant possessing Pi - 54 and the resultant intercross F 1 was selfed to generate intercross F 1 possessing the two blast and two BB resistance genes. The intercross F 1 s were then selfed to generate intercross F 2 s possessing different combination of resistance genes in homozygous condition including the four-gene pyramid lines. The homozygous lines were advanced further through pedigree method of selection. When selected gene-pyramid lines possessing both blast and BB resistance genes were screened against the two diseases, they exhibited high level of disease resistance. Further, all the gene-pyramid lines possessed grain type identical to ISM and yielded equivalent to or better than the original variety. This is the first report documenting the stacking of two major genes for blast ( Pi - 2  +  Pi - 54 ) and two major genes for BB ( Xa21  +  xa13 ) in rice through marker-assisted backcross breeding in a elite cultivar Improved Samba Mahsuri.
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ISSN:0014-2336
1573-5060
DOI:10.1007/s10681-016-1784-1