Search Results - "Rasor, Blake J"

An integrated in vivo/in vitro framework to enhance cell-free biosynthesis with metabolically rewired yeast extracts by Rasor, Blake J., Yi, Xiunan, Brown, Hunter, Alper, Hal S., Jewett, Michael C.

“…Cell-free systems using crude cell extracts present appealing opportunities for designing biosynthetic pathways and enabling sustainable chemical synthesis…”
Get full text

Cell-Free Protein Synthesis for High-Throughput Biosynthetic Pathway Prototyping by Rasor, Blake J, Vögeli, Bastian, Jewett, Michael C, Karim, Ashty S

“…Biological systems provide a sustainable and complimentary approach to synthesizing useful chemical products. Metabolic engineers seeking to establish…”
Get more information

Stepping on the Gas to a Circular Economy: Accelerating Development of Carbon-Negative Chemical Production from Gas Fermentation by Fackler, Nick, Heijstra, Björn D, Rasor, Blake J, Brown, Hunter, Martin, Jacob, Ni, Zhuofu, Shebek, Kevin M, Rosin, Rick R, Simpson, Séan D, Tyo, Keith E, Giannone, Richard J, Hettich, Robert L, Tschaplinski, Timothy J, Leang, Ching, Brown, Steven D, Jewett, Michael C, Köpke, Michael

“…Owing to rising levels of greenhouse gases in our atmosphere and oceans, climate change poses significant environmental, economic, and social challenges…”
Get full text

Development of Next Generation Synthetic Biology Tools for Use in Streptomyces venezuelae by Phelan, Ryan M, Sachs, Daniel, Petkiewicz, Shayne J, Barajas, Jesus F, Blake-Hedges, Jacquelyn M, Thompson, Mitchell G, Reider Apel, Amanda, Rasor, Blake J, Katz, Leonard, Keasling, Jay D

“…Streptomyces have a rich history as producers of important natural products and this genus of bacteria has recently garnered attention for its potential…”
Get full text

Analysis of the Innovation Trend in Cell-Free Synthetic Biology by Meyer, Conary, Nakamura, Yusuke, Rasor, Blake J., Karim, Ashty S., Jewett, Michael C., Tan, Cheemeng

“…Cell-free synthetic biology is a maturing field that aims to assemble biomolecular reactions outside cells for compelling applications in drug discovery,…”
Get full text

Lens development requires DNMT1 but takes place normally in the absence of both DNMT3A and DNMT3B activity by Hoang, Thanh V., Horowitz, Evan R., Chaffee, Blake R., Qi, Peipei, Flake, Rachel E., Bruney, Devin G., Rasor, Blake J., Rosalez, Savana E., Wagner, Brad D., Robinson, Michael L.

“…Despite the wealth of knowledge of transcription factors involved in lens development, little information exists about the role of DNA methylation in this…”
Get full text

Toward sustainable, cell-free biomanufacturing by Rasor, Blake J, Vögeli, Bastian, Landwehr, Grant M, Bogart, Jonathan W, Karim, Ashty S, Jewett, Michael C

“…[Display omitted] •Cell-free systems enable biochemical reactions in the absence of cell viability constraints.•Cell-free metabolic engineering presents…”
Get full text

Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale by Liew, Fungmin Eric, Nogle, Robert, Abdalla, Tanus, Rasor, Blake J., Canter, Christina, Jensen, Rasmus O., Wang, Lan, Strutz, Jonathan, Chirania, Payal, De Tissera, Sashini, Mueller, Alexander P., Ruan, Zhenhua, Gao, Allan, Tran, Loan, Engle, Nancy L., Bromley, Jason C., Daniell, James, Conrado, Robert, Tschaplinski, Timothy J., Giannone, Richard J., Hettich, Robert L., Karim, Ashty S., Simpson, Séan D., Brown, Steven D., Leang, Ching, Jewett, Michael C., Köpke, Michael

“…Many industrial chemicals that are produced from fossil resources could be manufactured more sustainably through fermentation. Here we describe the development…”
Get full text

In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design by Karim, Ashty S., Dudley, Quentin M., Juminaga, Alex, Yuan, Yongbo, Crowe, Samantha A., Heggestad, Jacob T., Garg, Shivani, Abdalla, Tanus, Grubbe, William S., Rasor, Blake J., Coar, David N., Torculas, Maria, Krein, Michael, Liew, FungMin (Eric), Quattlebaum, Amy, Jensen, Rasmus O., Stuart, Jeffrey A., Simpson, Sean D., Köpke, Michael, Jewett, Michael C.

“…The design and optimization of biosynthetic pathways for industrially relevant, non-model organisms is challenging due to transformation idiosyncrasies,…”
Get full text

Cell-free styrene biosynthesis at high titers by Grubbe, William S., Rasor, Blake J., Krüger, Antje, Jewett, Michael C., Karim, Ashty S.

“…Styrene is an important petroleum-derived molecule that is polymerized to make versatile plastics, including disposable silverware and foamed packaging…”
Get full text

Establishing a versatile toolkit of flux enhanced strains and cell extracts for pathway prototyping by Yi, Xiunan, Rasor, Blake J., Boadi, Nathalie, Louie, Katherine, Northen, Trent R., Karim, Ashty S., Jewett, Michael C., Alper, Hal S.

“…Building and optimizing biosynthetic pathways in engineered cells holds promise to address societal needs in energy, materials, and medicine, but it is often…”
Get full text

A dynamic kinetic model captures cell-free metabolism for improved butanol production by Martin, Jacob P., Rasor, Blake J., DeBonis, Jonathon, Karim, Ashty S., Jewett, Michael C., Tyo, Keith E.J., Broadbelt, Linda J.

“…Cell-free systems are useful tools for prototyping metabolic pathways and optimizing the production of various bioproducts. Mechanistically-based kinetic…”
Get full text

Cell Extracts from Bacteria and Yeast Retain Metabolic Activity after Extended Storage and Repeated Thawing by Rasor, Blake J., Karim, Ashty S., Alper, Hal S., Jewett, Michael C.

“…Cell-free synthetic biology enables rapid prototyping of biological parts and synthesis of proteins or metabolites in the absence of cell growth constraints…”
Get full text

At-Home, Cell-Free Synthetic Biology Education Modules for Transcriptional Regulation and Environmental Water Quality Monitoring by Jung, Jaeyoung K., Rasor, Blake J., Rybnicky, Grant A., Silverman, Adam D., Standeven, Janet, Kuhn, Robert, Granito, Teresa, Ekas, Holly M., Wang, Brenda M., Karim, Ashty S., Lucks, Julius B., Jewett, Michael C.

“…As the field of synthetic biology expands, the need to grow and train science, technology, engineering, and math (STEM) practitioners is essential. However,…”
Get full text

Mechanistic Insights into Cell-Free Gene Expression through an Integrated -Omics Analysis of Extract Processing Methods by Rasor, Blake J., Chirania, Payal, Rybnicky, Grant A., Giannone, Richard J., Engle, Nancy L., Tschaplinski, Timothy J., Karim, Ashty S., Hettich, Robert L., Jewett, Michael C.

“…Cell-free systems derived from crude cell extracts have developed into tools for gene expression, with applications in prototyping, biosensing, and protein…”
Get full text

Enhancing control of cell-free metabolism through pH modulation by Karim, Ashty S, Rasor, Blake J, Jewett, Michael C

“…Abstract Engineering metabolism for the synthesis of bio-based products in non-model organisms can be challenging. One specific challenge is that biosynthetic…”
Get full text

Modular cell-free expression plasmids to accelerate biological design in cells by Karim, Ashty S, Liew, Fungmin Eric, Garg, Shivani, Vögeli, Bastian, Rasor, Blake J, Gonnot, Aislinn, Pavan, Marilene, Juminaga, Alex, Simpson, Séan D, Köpke, Michael, Jewett, Michael C

“…Industrial biotechnology aims to produce high-value products from renewable resources. This can be challenging because model microorganisms-organisms that are…”
Get full text

Toward sustainable, cell-free biomanufacturing by Rasor, Blake J., Vögeli, Bastian, Landwehr, Grant M., Bogart, Jonathan W., Karim, Ashty S., Jewett, Michael C.

“…Industrial biotechnology is an attractive approach to address the need for low-cost fuels and products from sustainable resources. Unfortunately, cells impose…”
Get full text

Enhancing control of cell-free metabolism through pH modulation by Karim, Ashty S., Rasor, Blake J., Jewett, Michael C.

“…Engineering metabolism for the synthesis of bio-based products in non-model organisms can be challenging. One specific challenge is that biosynthetic pathways…”
Get full text

Cell-free styrene biosynthesis at high titers by Grubbe, William S., Rasor, Blake J., Krüger, Antje, Jewett, Michael C., Karim, Ashty S.

“…Styrene is an important petroleum-derived molecule that is polymerized to make versatile plastics, including disposable silverware and foamed packaging…”
Get full text