Combination of resistance to potato late blight in foliage and tubers by intraspecific dihaploid protoplast fusion

Five dihaploid Solanum tuberosum genotypes, encompassing different levels of polygenically inherited resistance to potato late blight disease (Phytophthora infestans (Mont.) de Bary) in foliage and tubers, were used in four intraspecific protoplast fusion combinations. Vigorous growing putative hybr...

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Bibliographic Details
Published in:	Euphytica Vol. 102; no. 3; pp. 363 - 370
Main Authors:	Rasmussen, J.O. (Aarhus Univ. (Denmark). Dept. of Molecular and Structural Biology), Nepper, J.P, Kirk, H.G, Tolstrup, K, Rasmussen, O.S
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer 01-01-1998 Springer Nature B.V
Subjects:	Agronomy. Soil science and plant productions Biological and medical sciences DIHAPLOIDIA DIHAPLOIDIE DIHAPLOIDY DISEASE RESISTANCE Flowers & plants Foliage Fundamental and applied biological sciences. Psychology GENETIC RESISTANCE Genetics and breeding of economic plants Genotypes HIBRIDOS SOMATICOS HYBRIDE SOMATIQUE Hybrids Laboratory tests Pest resistance PHYTOPHTHORA INFESTANS Plant pathogens Potatoes RAPD RESISTANCE AUX MALADIES RESISTANCE GENETIQUE RESISTENCIA A LA ENFERMEDAD RESISTENCIA GENETICA SOLANUM TUBEROSUM SOMATIC HYBRIDS Varietal selection. Specialized plant breeding, plant breeding aims Melting Plant pathogen Solanum tuberosum Phycomycetes Plant leaf Hybridization Genetic determinism Fungi Disease resistance Diploidy Sensitivity resistance Random amplified polymorphic DNA Dicotyledones Protoplast Angiospermae Phytophthora infestans Tuber Somatic hybrid Spermatophyta Tuber plant Solanaceae Thallophyta
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Summary:	Five dihaploid Solanum tuberosum genotypes, encompassing different levels of polygenically inherited resistance to potato late blight disease (Phytophthora infestans (Mont.) de Bary) in foliage and tubers, were used in four intraspecific protoplast fusion combinations. Vigorous growing putative hybrid calli were selected four weeks after electrofusion. Intact plants were regenerated from 6% of the selected calli. Verification of hybridity was accomplished by use of RAPD analysis which revealed that 53% of the regenerated plants were true somatic hybrids. The score of true somatic hybrids in the different fusion combinations ranged within 21% - 100%. The hybrid plants were analysed for resistance to foliage blight in a field trial and assessed for tuber blight resistance by use of a laboratory test. Resistance to late blight in foliage and tubers varied between the hybrids. Very high levels of resistance to both foliage and tuber blight were obtained in some hybrids. However, loss of resistance in some hybrids as compared to the parental plants were also observed. Possible reasons for the phenotypic disappearance of resistance to either foliage or tuber blight are suggested.[PUBLICATION ABSTRACT]
Bibliography:	F30 1999000421 H20 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0014-2336 1573-5060
DOI:	10.1023/A:1018302103100