Anti-proliferative activity of a purified polysaccharide isolated from the basidiomycete fungus Paxillus involutus

Anti-proliferative activity of a purified polysaccharide isolated from the basidiomycete fungus Paxillus involutus

•Polysaccharide (GIPinv) with anti-cancer activity was purified from Paxillus involutus.•GIPinv is 229kDa and is a heteroglycan.•GIPinv has mixed linkages and a degree of branching of 0.38.•GIPinv exerts its anti-proliferative activity through induction of apoptosis.•GIPinv inhibits growth of seven...

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Bibliographic Details
Published in:Carbohydrate polymers Vol. 181; pp. 923 - 930
Main Authors: Barad, Ankush, Mackedenski, Sebastian, Li, Wai Ming, Li, Xiao Jie, Lim, Bryan Chu Chwen, Rashid, Faran, Tackaberry, Linda E., Massicotte, Hugues B., Egger, Keith N., Reimer, Kerry, Cheung, Peter C.K., Lee, Chow H.
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-02-2018
Subjects:
British Columbia
Growth inhibition
Mushroom
Paxillus involutus
Polysaccharide
Paxillus involutus
British Columbia
Growth inhibition
Polysaccharide
Mushroom
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Summary:•Polysaccharide (GIPinv) with anti-cancer activity was purified from Paxillus involutus.•GIPinv is 229kDa and is a heteroglycan.•GIPinv has mixed linkages and a degree of branching of 0.38.•GIPinv exerts its anti-proliferative activity through induction of apoptosis.•GIPinv inhibits growth of seven different cancer cell lines. A growth-inhibitory polysaccharide (GIPinv) was purified using size-exclusion and ion-exchange chromatography from the fourth sodium hydroxide extraction step of a fungus found in British Columbia. The fungus was genetically identified as a member of the Paxillus involutus complex. GIPinv has an average molecular weight of 229kDa and is a heteroglycan composed of glucose (65.9%), galactose (20.8%), mannose (7.8%), fucose (3.2%) and xylose (2.3%). GC–MS methylation analysis suggests that GIPinv has mixed linkages in the backbone containing (1→6)-Gal (25.5%), (1→4)-Glc (18.3%), (1→6)-Glc (8.3%), (1→3)-Glc (5.3%) and (1→2)-Xyl (4.5%). GIPinv has branching points at (1→2, 6)-Man (8.6%) and (1→3, 6)-Man (4.9%) having unsubstituted fucose (8.3%) and glucose (16.3%) as terminal sugars. GIPinv had growth-inhibitory activity against several cancer cell lines and triggered apoptosis. GIPinv should be further explored as a potential anti-cancer agent and a unique polysaccharide.
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ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2017.11.058