Molecular characterization, computational analysis and expression profiling of Dmrt1 gene in Indian major carp, Labeo rohita (Hamilton 1822)

Molecular characterization, computational analysis and expression profiling of Dmrt1 gene in Indian major carp, Labeo rohita (Hamilton 1822)

Sexual dimorphism of fish morphology, physiology and behavior is diverse and complex in nature. Doublesex and mab-3 related transcription factor (Dmrt) is a large protein family whose function is sexual development and differentiation in vertebrates. Here, we report a full-length cDNA sequence of La...

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Bibliographic Details
Published in:Animal biotechnology Vol. 32; no. 4; pp. 413 - 426
Main Authors: Sahoo, L., Sahoo, S., Mohanty, M., Sankar, M., Dixit, S., Das, P., Rasal, K. D., Rather, M. A., Sundaray, J. K.
Format: Journal Article
Language:English
Published: England Taylor & Francis 02-08-2021
Taylor & Francis Ltd
Subjects:
Amino Acids
Animals
Aquaculture
Carps - genetics
Computer applications
Deoxyribonucleic acid
Differentiation
DM domain
DMRT1
DNA
Fish Proteins - genetics
Gene expression
gonadal maturation
Gravy
Labeo rohita
Male
Molecular docking
Molecular Docking Simulation
Molecular weight
Morphology
Phylogeny
Proteins
Reproduction (biology)
Sex differentiation
Sexual dimorphism
Testis
Transcription Factors - genetics
Transcriptome
Vertebrates
gonadal maturation
DM domain
DMRT1
Labeo rohita
Sex differentiation
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Summary:Sexual dimorphism of fish morphology, physiology and behavior is diverse and complex in nature. Doublesex and mab-3 related transcription factor (Dmrt) is a large protein family whose function is sexual development and differentiation in vertebrates. Here, we report a full-length cDNA sequence of Labeo rohita (rohu) Dmrt1 of 907 bp length having 798 bp of open reading frame encoding 265 amino acids. The molecular weight of rohu DMRT1 protein was found to be 28.74 KDa and isoelectric point was 7.53. DMRT1 protein contains 23 positively and 24 negatively charged amino acids with a GRAVY score of -0.618. A characteristic DM domain was found in DMRT1 protein, which is a novel DNA-binding domain. Phylogenetic analysis showed maximum similarity with Cyprinus carpio when compared with DMRT1 of other vertebrates. Molecular docking study identified active sites to be targeted for drug designing. Rohu DMRT1 was observed to interact with other proteins such as FOXL2, CYP19a1a, AMH and SOX9a. Differential expression study revealed higher expression in testis tissue implying its role in male sex differentiation and testicular development. The information generated in the present work could facilitate further research to resolve the issues related to gonadal maturation and reproduction of commercially important aquaculture species.
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ISSN:1049-5398
1532-2378
DOI:10.1080/10495398.2019.1707683