Search Results - "Rao, Nalam Madhusudhana"

Substrate structure and computation guided engineering of a lipase for omega-3 fatty acid selectivity by Moharana, Tushar Ranjan, Rao, Nalam Madhusudhana

“…Enrichment of omega-3 fatty acids (ɷ-3 FAs) in natural oils is important to realize their health benefits. Lipases are promising catalysts to perform this…”
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Engineering the loops in a lipase for stability in DMSO by Yedavalli, Poornima, Madhusudhana Rao, Nalam

“…Nearly 65% of the surface of a lipase, from Bacillus subtilis, is occupied by the loops. Since the loops are dynamic components of a protein, located on the…”
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Selective Enrichment of Omega-3 Fatty Acids in Oils by Phospholipase A1 by Ranjan Moharana, Tushar, Byreddy, Avinesh R, Puri, Munish, Barrow, Colin, Rao, Nalam Madhusudhana

“…Omega fatty acids are recognized as key nutrients for healthier ageing. Lipases are used to release ω-3 fatty acids from oils for preparing enriched ω-3 fatty…”
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Novel Series of Non-Glycerol-Based Cationic Transfection Lipids for Use in Liposomal Gene Delivery by Banerjee, Rajkumar, Das, Prasanta Kumar, Srilakshmi, Gollapudi Venkata, Chaudhuri, Arabinda, Rao, Nalam Madhusudhana

“…A novel series of nontoxic and non-glycerol-based simple monocationic transfection lipids containing one or two hydroxyethyl groups directly linked to the…”
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Role of active site rigidity in activity: MD simulation and fluorescence study on a lipase mutant by Kamal, Md Zahid, Mohammad, Tabrez Anwar Shamim, Krishnamoorthy, G, Rao, Nalam Madhusudhana

“…Relationship between stability and activity of enzymes is maintained by underlying conformational flexibility. In thermophilic enzymes, a decrease in…”
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Combinatorial Labeling Method for Improving Peptide Fragmentation in Mass Spectrometry by Kuchibhotla, Bhanuramanand, Kola, Sankara Rao, Medicherla, Jagannadham V., Cherukuvada, Swamy V., Dhople, Vishnu M., Nalam, Madhusudhana Rao

“…Annotation of peptide sequence from tandem mass spectra constitutes the central step of mass spectrometry-based proteomics. Peptide mass spectra are obtained…”
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Mutational probing of protein aggregates to design aggregation‐resistant proteins by Kamal, Mohamad Zahid, Kumar, Virender, Satyamurthi, Kundarapu, Das, Kushal Kumar, Rao, Nalam Madhusudhana

“…Characterization of amorphous protein aggregates may offer insights into the process of aggregation. Eleven single amino acid mutants of lipase (LipA of…”
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Draft Genome Sequence of Entomopathogenic Brevibacillus laterosporus Strain Lak 1210, an Alkaliphilic Chitin Degrader by Prasanna, Lakshmi, Moharana, Tushar Ranjan, Sheikh, Nizamuddin, Arravapalli, Veerabhadra Rao, Kumaraswamy, Thangaraj, Eijsink, Vincent G H, Nalam, Madhusudhana Rao

“…We announce here the draft genome sequence of strain Lak 1210, isolated from mangrove soil. This alkaliphilic strain is an efficient chitin degrader and has…”
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Recombinant fusion proteins TAT-Mu, Mu and Mu-Mu mediate efficient non-viral gene delivery by Rajagopalan, Rukkumani, Xavier, Jennifer, Rangaraj, Nandini, Rao, Nalam Madhusudhana, Gopal, Vijaya

“…Background The inherent ability of certain peptides or proteins of viral, prokaryotic and eukaryotic origin to bind DNA was used to generate novel…”
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Quantitative aspects of endocytic activity in lipid-mediated transfections by Prasad, Tekkatte Krishnamurthy, Rangaraj, Nandini, Rao, Nalam Madhusudhana

“…Variation in transfection efficiency observed in different cell-types is poorly understood. To investigate the influence of endocytic activity on…”
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Design, Synthesis, and Transfection Biology of Novel Cationic Glycolipids for Use in Liposomal Gene Delivery by Banerjee, Rajkumar, Mahidhar, Yenugonda Venkata, Chaudhuri, Arabinda, Gopal, Vijaya, Rao, Nalam Madhusudhana

“…The molecular structure of the cationic lipids used in gene transfection strongly influences their transfection efficiency. High transfection efficiencies of…”
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Anchor Dependency for Non-Glycerol Based Cationic Lipofectins: Mixed Bag of Regular and Anomalous Transfection Profiles by Singh, Rajkumar Sunil, Mukherjee, Koushik, Banerjee, Rajkumar, Chaudhuri, Arabinda, Hait, Samik Kumar, Moulik, Satya Priya, Ramadas, Yerramsetti, Vijayalakshmi, Amash, Rao, Nalam Madhusudhana

“…Although detailed structure–activity, physicochemical and biophysical investigations in probing the anchor influence in liposomal gene delivery have been…”
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Haloperidol-associated Stealth Liposomes by Mukherjee, Amarnath, Prasad, Tekkatte Krishnamurthy, Rao, Nalam Madhusudhana, Banerjee, Rajkumar

“…Sigma receptors are membrane-bound proteins that are overexpressed in certain human malignancies including breast cancer. These receptors show very high…”
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X-ray structure and characterization of a thermostable lipase from Geobacillus thermoleovorans by Moharana, Tushar Ranjan, Pal, Biswajit, Rao, Nalam Madhusudhana

“…Thermo-alkalophilic bacterium, Geobacillus thermoleovorans secrets many enzymes including a 43 kDa extracellular lipase. Significant thermostability, organic…”
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Lipase in aqueous‐polar organic solvents: Activity, structure, and stability by Kamal, Md. Zahid, Yedavalli, Poornima, Deshmukh, Mandar V., Rao, Nalam Madhusudhana

“…Studying alterations in biophysical and biochemical behavior of enzymes in the presence of organic solvents and the underlying cause(s) has important…”
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Thermally denatured state determines refolding in lipase: Mutational analysis by Ahmad, Shoeb, Rao, Nalam Madhusudhana

“…Irreversibility of thermally denatured proteins due to aggregation limits thermodynamic characterization of proteins and also confounds the identification of…”
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A computational search for lipases that can preferentially hydrolyze long-chain omega-3 fatty acids from fish oil triacylglycerols by Kamal, Md. Zahid, Barrow, Colin J., Rao, Nalam Madhusudhana

“…•Omega-3 fatty acid containing triglycerides were docked on all lipases with known structure.•Both non-covalent and covalent docking experiments were…”
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In Vitro Evolved Non-Aggregating and Thermostable Lipase: Structural and Thermodynamic Investigation by Kamal, Md. Zahid, Ahmad, Shoeb, Molugu, Trivikram Rao, Vijayalakshmi, Amash, Deshmukh, Mandar V., Sankaranarayanan, Rajan, Rao, Nalam Madhusudhana

“…Rational and in vitro evolutionary approaches to improve either protein stability or aggregation resistance were successful, but empirical rules for…”
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Tween 80 influences the production of intracellular lipase by Schizochytrium S31 in a stirred tank reactor by Byreddy, Avinesh R., Rao, Nalam Madhusudhana, Barrow, Colin J., Puri, Munish

“…[Display omitted] •Tween 80 influenced lipase productivity in Schizochytrium S31.•Sonication was optimised for the extraction of intracellular lipase from…”
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Efficiency of dual siRNA-mediated gene therapy for intervertebral disc degeneration (IVDD) by Banala, Rajkiran Reddy, Vemuri, Satish Kumar, Dar, Ghulam Hassan, Palanisamy, Vijayanand, Penkulinti, Murahari, Surekha, MV, Gurava Reddy, AV, Nalam, Madhusudhana Rao, Subbaiah, GPV

“…One of the common causes of low back pain is intervertebral disc degeneration. The pathophysiology of disc degeneration involves apoptosis of nucleus pulposes…”
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