Genetic resources offer efficient tools for rice functional genomics research

Genetic resources offer efficient tools for rice functional genomics research

Summary statement Compared with the robustness of genome sequencing and bioinformatics techniques, progress in understanding the function of rice genes has lagged, hampering the utilization of rice genes for cereal crop improvement. In this review, we discuss the important features of existing T‐DNA...

Full description

Saved in:
Bibliographic Details
Published in:Plant, cell and environment Vol. 39; no. 5; pp. 998 - 1013
Main Authors: Lo, Shuen‐Fang, Fan, Ming‐Jen, Hsing, Yue‐Ie, Chen, Liang‐Jwu, Chen, Shu, Wen, Ien‐Chie, Liu, Yi‐Lun, Chen, Ku‐Ting, Jiang, Mirng‐Jier, Lin, Ming‐Kuang, Rao, Meng‐Yen, Yu, Lin‐Chih, Ho, Tuan‐Hua David, Yu, Su‐May
Format: Journal Article
Language:English
Published: United States Wiley Subscription Services, Inc 01-05-2016
Subjects:
functional genomics
gene activation
gene knockout
Gene Knockout Techniques
Genomics - methods
Mutation - genetics
Oryza - genetics
promoter trapping
Reverse Genetics
rice
T‐DNA
gene knockout
functional genomics
T-DNA
promoter trapping
rice
gene activation
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary statement Compared with the robustness of genome sequencing and bioinformatics techniques, progress in understanding the function of rice genes has lagged, hampering the utilization of rice genes for cereal crop improvement. In this review, we discuss the important features of existing T‐DNA activation‐ and knockout‐tagged rice mutant populations, summarize the recent progress in functional genomics research using these resources and discuss how to more effectively utilize these resources to expedite functional genomics and phenomics studies following forward and reverse genetics approaches. These studies utilizing the T‐DNA insertion mutant populations may facilitate the translation of rice functional genomics research to improvements of rice and other cereal crops. Rice is an important crop and major model plant for monocot functional genomics studies. With the establishment of various genetic resources for rice genomics, the next challenge is to systematically assign functions to predicted genes in the rice genome. Compared with the robustness of genome sequencing and bioinformatics techniques, progress in understanding the function of rice genes has lagged, hampering the utilization of rice genes for cereal crop improvement. The use of transfer DNA (T‐DNA) insertional mutagenesis offers the advantage of uniform distribution throughout the rice genome, but preferentially in gene‐rich regions, resulting in direct gene knockout or activation of genes within 20–30 kb up‐ and downstream of the T‐DNA insertion site and high gene tagging efficiency. Here, we summarize the recent progress in functional genomics using the T‐DNA‐tagged rice mutant population. We also discuss important features of T‐DNA activation‐ and knockout‐tagging and promoter‐trapping of the rice genome in relation to mutant and candidate gene characterizations and how to more efficiently utilize rice mutant populations and datasets for high‐throughput functional genomics and phenomics studies by forward and reverse genetics approaches. These studies may facilitate the translation of rice functional genomics research to improvements of rice and other cereal crops.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0140-7791
1365-3040
DOI:10.1111/pce.12632