Early osmotic, antioxidant, ionic, and redox responses to salinity in leaves and roots of Indian mustard (Brassica juncea L.)

Salt-stress-induced alterations in osmotic, ionic, and redox responses were studied in the early period of treatment (30 min to 5 days) in seedlings of Brassica juncea L. Roots and shoots under mild (50 mM) and severe (250 mM) NaCl stress were analyzed for growth, oxidative stress, osmolyte accumula...

Full description

Saved in:

Bibliographic Details
Published in:	Protoplasma Vol. 253; no. 1; pp. 101 - 110
Main Authors:	Ranjit, Singh Laxmi, Manish, Pandey, Penna, Suprasanna
Format:	Journal Article
Language:	English
Published:	Vienna Springer Vienna 01-01-2016 Springer Nature B.V
Subjects:	antioxidant activity antioxidants Antioxidants - metabolism Biomass Biomedical and Life Sciences Brassica juncea Cell Biology enzyme activity glutathione growth retardation hydrogen peroxide Hydrogen Peroxide - metabolism Ions - metabolism leaves Life Sciences Mustard Plant - drug effects Mustard Plant - metabolism Original Article Osmosis Oxidation-Reduction oxidative stress Plant Leaves - drug effects Plant Leaves - metabolism Plant Roots - drug effects Plant Roots - growth & development Plant Roots - metabolism Plant Sciences Plant Shoots - drug effects Plant Shoots - growth & development Principal Component Analysis roots Salinity salt stress seedlings Seedlings - drug effects Seedlings - metabolism shoots sodium sodium chloride Sodium Chloride - pharmacology Stress, Physiological - drug effects superoxide dismutase Water - metabolism water content Zoology Roots and shoots Salt stress Antioxidant enzymes H Early events O Sodium-potassium Brassica H2O2
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Salt-stress-induced alterations in osmotic, ionic, and redox responses were studied in the early period of treatment (30 min to 5 days) in seedlings of Brassica juncea L. Roots and shoots under mild (50 mM) and severe (250 mM) NaCl stress were analyzed for growth, oxidative stress, osmolyte accumulation, antioxidant defense, and redox state. Growth reduction was less pronounced in the early time period of salt stress while oxidative damage increased linearly and in a sustained manner under severe stress up to 6 h. An early and transient reactive oxygen species (ROS) burst, as evidenced by superoxide and hydrogen peroxide level was observed, followed by activation of enzymatic antioxidant system (GPX, SOD, CAT, and GR) in both root and shoot. The enzymatic activity was not affected much under mild stress particularly at early phase; however, severe stress induced a significant increase in the activity of antioxidant enzymes. Root ascorbate was progressively accumulated, and its redox state maintained in the early time phase of treatment under mild stress while increase in root and shoot glutathione content was recorded under mild stress at 5 days when the active ascorbate pool decreased. While early period of salt stress showed significant Na⁺ accumulation over control, plants subjected to mild stress measured less Na⁺ accumulation up to 5 days compared to severely stressed plants. The results showed an early induction of differential responses to salt stress in roots and shoots of Brassica which include growth limitations, reduced relative water content, increased osmolytes, redox state, and antioxidant system, and a significant Na⁺ increase. The results also indicate that roots and shoots may have distinct mechanisms of responses to salt stress.
Bibliography:	http://dx.doi.org/10.1007/s00709-015-0792-7 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0033-183X 1615-6102
DOI:	10.1007/s00709-015-0792-7