Virtual screening and discovery of novel aurora kinase inhibitors

Cancer remains one of the major contributors to human mortality and a hazard to human growth. The search for a new treatment continues unabated. Aurora kinases play an important role in cell cycle, and thus a potential target for the treatment of cancer. In the present work, we aim to discover poten...

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Bibliographic Details
Published in:	Current topics in medicinal chemistry Vol. 14; no. 17; p. 2006
Main Authors:	Raghu, R, Devaraji, Vinod, Leena, K, Riyaz, S D, Rani, Polavaru Baby, Kumar, B Suneel, Naik, Pradeep Kumar, Dubey, P K, Velmurugan, Devadasan, Vijayalakshmi, M
Format:	Journal Article
Language:	English
Published:	United Arab Emirates 01-01-2014
Subjects:	Aurora Kinases - antagonists & inhibitors Aurora Kinases - metabolism Benzopyrans - chemical synthesis Benzopyrans - chemistry Benzopyrans - pharmacology Breast Neoplasms - drug therapy Cell Line, Tumor Colorectal Neoplasms - drug therapy Female Humans Indoles - chemical synthesis Indoles - chemistry Indoles - pharmacology Inhibitory Concentration 50 Magnetic Resonance Spectroscopy Mass Spectrometry Models, Molecular Molecular Docking Simulation Protein Kinase Inhibitors - chemistry Protein Kinase Inhibitors - pharmacology Protein Kinase Inhibitors - therapeutic use Spectrophotometry, Infrared
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Summary:	Cancer remains one of the major contributors to human mortality and a hazard to human growth. The search for a new treatment continues unabated. Aurora kinases play an important role in cell cycle, and thus a potential target for the treatment of cancer. In the present work, we aim to discover potential leads against aurora kinase using various rational methods of drug discovery. The available crystal complexes of AKs were analyzed for their interactions and quantified with glide-extra precision (XP) docking. About 20 crystal pdb were selected from the protein databank based on the resolution factor, R-factor and R-value. And after docking with the native ligands, the RMSD value was calculated, wherein the protein with the least RMSD was found to be 3UOK which was further used for our screening of small molecules from the in-house database by molecular docking. Fragments which were found to possess the best interactions were considered for the synthesis with characterization, and biological activity was carried out against breast cancer and colorectal cancer cell lines to assess the inhibitory capability of synthesized compounds. Molecule with the molecular id IS2 i.e. (3E)-3-(5-fluoro-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-2H chromene-2,4(3H)-dione was found to possess inhibitory activity with an IC50 of 1.324 nM and 5.785 µM for breast cell line and colorectal cell line studies, respectively.
ISSN:	1873-4294
DOI:	10.2174/1568026614666140929151140