Genome mapping of three major resistance genes to woolly apple aphid (Eriosoma lanigerum Hausm.)

Genome mapping of three major resistance genes to woolly apple aphid (Eriosoma lanigerum Hausm.)

Woolly apple aphid (WAA; Eriosoma lanigerum Hausm.) can be a major economic problem to apple growers in most parts of the world, and resistance breeding provides a sustainable means to control this pest. We report molecular markers for three genes conferring WAA resistance and placing them on two li...

Full description

Saved in:
Bibliographic Details
Published in:Tree genetics & genomes Vol. 4; no. 2; pp. 223 - 236
Main Authors: Bus, V. G. M, Chagné, D, Bassett, H. C. M, Bowatte, D, Calenge, F, Celton, J.-M, Durel, C.-E, Malone, M. T, Patocchi, A, Ranatunga, A. C, Rikkerink, E. H. A, Tustin, D. S, Zhou, J, Gardiner, S. E
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01-04-2008
Springer-Verlag
Springer Nature B.V
Springer Verlag
Subjects:
Agricultural sciences
Aphididae
Apples
Biomedical and Life Sciences
Biotechnology
Cultivars
Deoxyribonucleic acid
DNA
Eriosoma lanigerum
Forestry
Fruits
Gene mapping
Genetic map
Genetic markers
Genetics
Genomes
Genomics
Life Sciences
Malus
Original Paper
Pest control
Pests
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Resistance gene
Silviculture, forestry
Tree Biology
Genetic markers
Woolly apple aphid
Resistance gene
Genetic map
WOLLY APPLE APHID
ERIOSOMA LANIGERUM
GENETIC MAP
ER GENE
GENETIC MARKERS
MARQUEURS GENETIQUES
RESISTANCE GENE
MALUS
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Woolly apple aphid (WAA; Eriosoma lanigerum Hausm.) can be a major economic problem to apple growers in most parts of the world, and resistance breeding provides a sustainable means to control this pest. We report molecular markers for three genes conferring WAA resistance and placing them on two linkage groups (LG) on the genetic map of apple. The Er1 and Er2 genes derived from 'Northern Spy' and 'Robusta 5,' respectively, are the two major genes that breeders have used to date to improve the resistance of apple rootstocks to this pest. The gene Er3, from 'Aotea 1' (an accession classified as Malus sieboldii), is a new major gene for WAA resistance. Genetic markers linked to the Er1 and Er3 genes were identified by screening random amplification of polymorphic deoxyribonucleic acid (DNA; RAPD) markers across DNA bulks from resistant and susceptible plants from populations segregating for these genes. The closest RAPD markers were converted into sequence-characterized amplified region markers and the genome location of these two genes was assigned to LG 08 by aligning the maps around the genes with a reference map of 'Discovery' using microsatellite markers. The Er2 gene was located on LG 17 of 'Robusta 5' using a genetic map developed in a M.9 x 'Robusta 5' progeny. Markers for each of the genes were validated for their usefulness for marker-assisted selection in separate populations. The potential use of the genetic markers for these genes in the breeding of apple cultivars with durable resistance to WAA is discussed.
Bibliography:http://dx.doi.org/10.1007/s11295-007-0103-3
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
ISSN:1614-2942
1614-2950
DOI:10.1007/s11295-007-0103-3