Structural studies of the O-antigen polysaccharide from Escherichia coli O115 and biosynthetic aspects thereof

Structural studies of the O-antigen polysaccharide from Escherichia coli O115 and biosynthetic aspects thereof

The structure of the O-antigen polysaccharide (PS) of Escherichia coli O115 has been investigated using a combination of component analysis and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy experiments. The repeating unit of the O-antigen was elucidated using the O-deacetylated PS and has...

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Bibliographic Details
Published in:Glycobiology (Oxford) Vol. 23; no. 3; pp. 354 - 362
Main Authors: Fontana, Carolina, Ramström, Kristoffer, Weintraub, Andrej, Widmalm, Göran
Format: Journal Article
Language:English
Published: England 01-03-2013
Subjects:
Acetylglucosamine - chemistry
Acetyltransferase
Acetyltransferases - genetics
Carbohydrate Conformation
Carbohydrate Sequence
Escherichia coli
Escherichia coli - chemistry
Escherichia coli - enzymology
Escherichia coli - genetics
Gene clusters
Glycosyltransferase
glycosyltransferases
Glycosyltransferases - genetics
lipopolysaccharide
Magnetic Resonance Spectroscopy
Medicin och hälsovetenskap
Multigene Family
N-Acetyl-D-glucosamine
N.M.R
O Antigens - biosynthesis
O Antigens - chemistry
O-acetylation
O-antigen
Organic Chemistry
organisk kemi
Polysaccharides
Probes
Sequence Analysis, DNA
Shigella dysenteriae
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Summary:The structure of the O-antigen polysaccharide (PS) of Escherichia coli O115 has been investigated using a combination of component analysis and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy experiments. The repeating unit of the O-antigen was elucidated using the O-deacetylated PS and has the following branched pentasaccharide structure: →3)[β-L-Rhap-(1 → 4)]-β-D-GlcpNAc-(1 → 4)-α-D-GalpA-(1 → 3)-α-D-Manp-(1 → 3)-β-D-GlcpNAc-(1→. Cross-peaks of low intensity, corresponding to a β-L-Rhap-(1 → 4)-β-D-GlcpNAc-(1→ structural element, were present in the NMR spectra and attributed to the terminal part of the PS; this information defines the biological repeating unit of the O-antigen by having a 3-substituted N-acetyl-D-glucosamine (GlcNAc) residue at its reducing end. Analysis of the NMR spectra of the native PS revealed O-acetyl groups distributed over different positions of the l-Rhap residue (∼0.70 per repeating unit) as well as at O-2 and O-3 of the D-GalpA residue (∼0.03 and ∼0.25 per repeating unit, respectively), which is in agreement with the presence of two acetyltransferases previously identified in the O-antigen gene cluster (Wang Q, Ruan X, Wei D, Hu Z, Wu L, Yu T, Feng L, Wang L. 2010. Mol Cell Probes. 24:286-290.). In addition, the four glycosyltransferases initially identified in the O-antigen gene cluster of E. coli O115 were analyzed using BLAST, and the function of two of them predicted on the basis of similarities with glycosyltransferases from Shigella dysenteriae type 5 and 12, as well as E. coli O58 and O152.
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ISSN:0959-6658
1460-2423
1460-2423
DOI:10.1093/glycob/cws161