Equine Parvovirus-Hepatitis Screening in Horses and Donkeys with Histopathologic Liver Abnormalities

There is strong evidence that equine parvovirus-hepatitis (EqPV-H) is associated with the onset of Theiler's disease, an acute hepatic necrosis, in horses. However, the impact of this virus on other hepatopathies remains unknown. The objective of this retrospective study was to evaluate the pre...

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Bibliographic Details
Published in:	Viruses Vol. 13; no. 8; p. 1599
Main Authors:	Zehetner, Verena, Cavalleri, Jessika-M V, Klang, Andrea, Hofer, Martin, Preining, Irina, Steinborn, Ralf, Ramsauer, Anna S
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 12-08-2021 MDPI
Subjects:	Animals Chronic infection Cirrhosis Disease EqPV-H Equidae - virology Female Genomes Hepatitis Hepatitis Viruses - genetics Hepatitis, Viral, Animal - diagnosis Hepatitis, Viral, Animal - epidemiology hepatopathy horse Horse Diseases - diagnosis Horse Diseases - virology Horses Horses - virology Inflammatory diseases Liver Liver - pathology Liver - virology Liver cirrhosis Liver diseases Liver Diseases - diagnosis Liver Diseases - epidemiology Liver Diseases - veterinary Liver Diseases - virology Male Mass Screening - veterinary Metabolism Metastases neoplasia Paraffin Parvoviridae Infections - diagnosis Parvoviridae Infections - epidemiology Parvovirus - genetics Parvovirus - isolation & purification Parvoviruses Persistent Infection - diagnosis Persistent Infection - virology Polymerase chain reaction Real-Time Polymerase Chain Reaction Retrospective Studies Serologic Tests Standard deviation Tetanus ungulate copiparvovirus 6 Viral Load Austria France United States > US China Germany horse EqPV-H hepatopathy hepatitis neoplasia ungulate copiparvovirus 6
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Summary:	There is strong evidence that equine parvovirus-hepatitis (EqPV-H) is associated with the onset of Theiler's disease, an acute hepatic necrosis, in horses. However, the impact of this virus on other hepatopathies remains unknown. The objective of this retrospective study was to evaluate the prevalence and quantify the viral loads of EqPV-H in formalin-fixed, paraffin-embedded equine and donkey livers with various histopathologic abnormalities. The pathologies included cirrhosis, circulatory disorders of the liver, toxic and metabolic hepatic diseases as well as neoplastic and inflammatory diseases ( = 84). Eight normal liver samples were included for comparison as controls. EqPV-H DNA was qualitatively and quantitatively measured by real-time PCR and digital PCR, respectively. The virus was detected in two livers originating from horses diagnosed with abdominal neoplasia and liver metastasis (loads of 5 × 10 and 9.5 × 10 genome equivalents per million cells). The amount of viral nucleic acids measured indicates chronic infection or persistence of EqPV-H, which might have been facilitated by the neoplastic disease. In summary, this study did not provide evidence for EqPV-H being involved in hepatopathies other than Theiler's disease.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1999-4915 1999-4915
DOI:	10.3390/v13081599