Production of Pleurotus sajor-caju crude enzyme broth and its applicability for the removal of bisphenol A

Bisphenol A is an endocrine interfering compound, produced and used on a large scale worldwide. Chemical and biologic methods can be used to remove it from the environment. Biological methods are considered less costly, safer and, according to green chemistry definitions, an environmentally correct...

Full description

Saved in:

Bibliographic Details
Published in:	Anais da Academia Brasileira de Ciências Vol. 93; no. 1; p. e20191153
Main Authors:	Rampinelli, Jamile R, Melo, Mahara P DE, Arbigaus, Auriciane, Silveira, Márcia L L DA, Wagner, Theodoro M, Gern, Regina M M, Wisbeck, Elisabeth, Bonatti-Chaves, Mariane, Furigo Júnior, Agenor, Furlan, Sandra A
Format:	Journal Article
Language:	English
Published:	Brazil Academia Brasileira de Ciências 01-01-2021
Subjects:	Banana wastes Basidiomycetes Benzhydryl Compounds endocrine disruptor enzymatic activity Submerged culture Laccase Lentinula MULTIDISCIPLINARY SCIENCES Phenols Pleurotus Basidiomycetes endocrine disruptor Banana wastes enzymatic activity Submerged culture
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Bisphenol A is an endocrine interfering compound, produced and used on a large scale worldwide. Chemical and biologic methods can be used to remove it from the environment. Biological methods are considered less costly, safer and, according to green chemistry definitions, an environmentally correct method. Considering the use of a crude enzyme broth, without any downstream process, the costs could be mostly reduced. Thus, the removal of bisphenol A by Pleurotus sajor-caju crude enzyme broth was investigated. Initially, the agro-industrial wastes were characterized and, the composition of the culture medium and the bioreactor culture conditions were defined. The enzyme produced in the highest concentration was characterized and the crude broth used in the bisphenol A removal assays. The OXI45 culture medium presented the highest laccase activity (1,850.7 U L-1, 350 rpm). Greater laccase stability was observed at 20 - 40 oC and pHs 5 - 7. Vanillin and ferulic acid (considered mediator compounds) were identified in the crude broth, probably helping on the obtention of the high value of removal effectiveness (0.052 mg U-1 h-1). The results indicate the potential use of the Pleurotus sajor-caju crude enzyme broth to obtain an enzymatic formulation for application in the environmental area.
ISSN:	0001-3765 1678-2690 1678-2690
DOI:	10.1590/0001-3765202120191153