Dectin-1-Mediated Production of Pro-Inflammatory Cytokines Induced by Yeast β-Glucans in Bovine Monocytes

Yeast-derived products containing β-glucans have long been used as feed supplements in domesticated animals in an attempt to increase immunity. β-glucans are mainly recognized by the cell surface receptor CLEC7A, also designated Dectin-1. Although the immune mechanisms elicited through Dectin-1 acti...

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Bibliographic Details
Published in:	Frontiers in immunology Vol. 12; p. 689879
Main Authors:	Pedro, Ana R. V., Lima, Tânia, Fróis-Martins, Ricardo, Leal, Bárbara, Ramos, Isabel C., Martins, Elisabete G., Cabrita, Ana R. J., Fonseca, António J. M., Maia, Margarida R. G., Vilanova, Manuel, Correia, Alexandra
Format:	Journal Article
Language:	English
Published:	Frontiers Media S.A 28-05-2021
Subjects:	bovine cytokines dectin-1 Immunology monocytes siRNA β-glucans
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Summary:	Yeast-derived products containing β-glucans have long been used as feed supplements in domesticated animals in an attempt to increase immunity. β-glucans are mainly recognized by the cell surface receptor CLEC7A, also designated Dectin-1. Although the immune mechanisms elicited through Dectin-1 activation have been studied in detail in mice and humans, they are poorly understood in other species. Here, we evaluated the response of bovine monocytes to soluble and particulate purified β-glucans, and also to Zymosan. Our results show that particulate, but not soluble β-glucans, can upregulate the surface expression of costimulatory molecules CD80 and CD86 on bovine monocytes. In addition, stimulated cells increased production of IL-8 and of TNF, IL1B , and IL6 mRNA expression, in a dose-dependent manner, which correlated positively with CLEC7A gene expression. Production of IL-8 and TNF expression decreased significantly after CLEC7A knockdown using two different pairs of siRNAs. Overall, we demonstrated here that bovine monocytes respond to particulate β-glucans, through Dectin-1, by increasing the expression of pro-inflammatory cytokines. Our data support further studies in cattle on the induction of trained immunity using dietary β-glucans.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Comparative Immunology, a section of the journal Frontiers in Immunology Edited by: Nicolas Bertho, INRA Biologie, Épidémiologie et Analyse de Risque en santé animale (BIOEPAR), France Reviewed by: Karelle De Luca, Boehringer Ingelheim, France; Kristen Alicia Byrne, National Animal Disease Center (USDA ARS), United States
ISSN:	1664-3224 1664-3224
DOI:	10.3389/fimmu.2021.689879