Molecular Characterization of Arcobacter Isolates Using Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR)

In modern years, Arcobacters are reflected as potential emerging food-borne zoonotic entero-pathogens. Arcobacter species displayed a wide variety of genetic diversity. The study was carried out to genotype and find molecular heterogeneity of Arcobacter spp. (Arcobacter butzleri, A. cryaerophilus an...

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Bibliographic Details
Published in:	Asian journal of animal and veterinary advances Vol. 9; no. 9; pp. 543 - 555
Main Authors:	Bagalakote, P.S., Rathore, R.S., Ramees, T.P., Mohan, H.V., Sumankumar, M., Agarwal, R.K., Kumar, A., Dhama, K.
Format:	Journal Article
Language:	English
Published:	2014
Subjects:	Arcobacter Arcobacter butzleri Arcobacter cryaerophilus
Online Access:	Get full text
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Summary:	In modern years, Arcobacters are reflected as potential emerging food-borne zoonotic entero-pathogens. Arcobacter species displayed a wide variety of genetic diversity. The study was carried out to genotype and find molecular heterogeneity of Arcobacter spp. (Arcobacter butzleri, A. cryaerophilus and A. skirrowii), isolated from different sources from Bareilly region, Uttar Pradesh, India by using randomly amplified polymorphic DNA - polymerase chain reaction (RAPD-PCR). RAPD-PCR was performed using genomic DNA of Arcobacter isolates (n = 56; 33 A. butzleri, 20 A. cryaerophilus, 3 A. skirrowii; recovered from chicken meat, pork, sheep faeces, goat faeces, poultry intestinal contents and human diarrhoeal stool samples) as template by employing two published primers. The RAPD profiling for primer 1 (HLWL85) yielded number of bands rangeing between 2-8 (500-3100 bp). Out of 56 isolates, 54 showed bands giving a type ability of 96.4%. These 54 typable strains were grouped to 35 types and giving discriminatory power of 0.9762. Primer 2 (OPA-11) yielded RAPD-PCR profiles comprising of 2-7 bands (210-2800 bp). Out of total 56 isolates, 54 were typable with a discriminatory power of 0.9336. This is the first report from India regarding RAPD profiling of Arcobacter spp. This study reveals epidemiological relationship of Arcobacter isolate from various sources and will help to design suitable prevention and control strategies for this important pathogen having public health significance.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1683-9919 1996-3289
DOI:	10.3923/ajava.2014.543.555