Decrease in the platelet level of 43 kDa immunoreactive fraction of serotonin transporting protein correlates with depressive symptoms in patients with somatoform disorders

Decrease in the platelet level of 43 kDa immunoreactive fraction of serotonin transporting protein correlates with depressive symptoms in patients with somatoform disorders

To elucidate the role of serotonin transporter (SERT) protein as a candidate target for somatoform disorders (SD), we have performed an association study of the SERT immunoreactive (IR-SERT) protein level by using site-specific antibodies directed at C-terminus epitope poorly conserved among other c...

Full description

Saved in:
Bibliographic Details
Published in:Voprosy meditsinskoi khimii Vol. 45; no. 3; p. 256
Main Authors: Belous, A R, Ramamoothy, S, Blakely, R D, Faktor, M I, Lozier, R Kh, Dupin, A M, Bechiashvili, A G, Morozova, M A, Brusov, O S
Format: Journal Article
Language:Russian
Published: Russia (Federation) 01-05-1999
Subjects:
Adult
Blood Platelets - metabolism
Carrier Proteins - blood
Depression - blood
Depression - complications
Female
Humans
Immunoblotting
Male
Membrane Glycoproteins - blood
Membrane Transport Proteins
Middle Aged
Nerve Tissue Proteins
Serotonin - blood
Serotonin Plasma Membrane Transport Proteins
Somatoform Disorders - blood
Somatoform Disorders - complications
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To elucidate the role of serotonin transporter (SERT) protein as a candidate target for somatoform disorders (SD), we have performed an association study of the SERT immunoreactive (IR-SERT) protein level by using site-specific antibodies directed at C-terminus epitope poorly conserved among other cotransporters. 22 patients who met DSM-IY criteria for SD and 32 healthy volunteers matched in sex and age participated in the study. In platelets from all healthy donors, IR-SERT proteins migrates as a diffuse band between 75 and 102 kDa and a major sharper band at 43 kDa. We revealed almost complete disappearance of platelet 43 kDa IR-SERT protein in all patients with SD. All patients were rated with Hamilton Depressive Rating Scale (HDRS). There were found a significant negative correlation between residual IR-SERT 43 kDa protein level and HDRS Scores [r = -0.83; P < 0.000001]. These findings suggest that a biosynthetic or processing abnormality may exist in SERTs in the affected population and may reflect the dysfunction of serotonin neurotransmission in CNS.
ISSN:0042-8809