Application of BioFire FilmArray Blood Culture Identification panel for rapid identification of the causative agents of ventilator-associated pneumonia

To evaluate the ability of the BioFire FilmArray Blood Culture Identification (BCID) panel to rapidly detect pathogens producing late-onset ventilator-associated pneumonia (VAP), a severe infection often produced by Gram-negative bacteria. These microorganisms are frequently multidrug resistant and...

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Published in:	Clinical microbiology and infection Vol. 24; no. 11; pp. 1213.e1 - 1213.e4
Main Authors:	Pulido, M.R., Moreno-Martínez, P., González-Galán, V., Fernández Cuenca, F., Pascual, Á., Garnacho-Montero, J., Antonelli, M., Dimopoulos, G., Lepe, J.A., McConnell, M.J., Cisneros, J.M., Ramírez Gallmore, P., Bonastre, J., Montejo González, J.C., Díaz-Miguel, R.O., García, A.E., Crespo, R.Z., Camerino, R.S., Gutiérrez, M.H., Alvarez-Rocha, L., Sanchez Garcia, M., Allegue Gallego, J.M., Castellanos Ortega, Á., de la Torre Prados, M.V., Roca, R.F., Cortés, P.V., Armaganidis, A., Serafim, Georgopoulos, D., Pneumatikos, L., Nakos, G., Baltopoulos, G., Koutsoukou, A., Zakynthinos, E., Militsa-Bitsani, Komnos, A., Pennisi, M., De Pascale, G., Di Gravio, V., Rocco, M., De Blasi, R., De Gasperi, A., Ranieri- Francesco de Rosa, V.M., De Robertis-Rosalba, E., Guarracino
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-11-2018
Subjects:	Antibiotic resistance Bacteria - isolation & purification Bacteriological Techniques - methods Blood Culture - methods Female FilmArray BCID panel Gram-negative bacteria Humans Male Pneumonia, Bacterial - microbiology Pneumonia, Ventilator-Associated - diagnosis Pneumonia, Ventilator-Associated - microbiology Rapid diagnosis Ventilator-associated pneumonia Rapid diagnosis Ventilator-associated pneumonia Gram-negative bacteria FilmArray BCID panel Antibiotic resistance
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Summary:	To evaluate the ability of the BioFire FilmArray Blood Culture Identification (BCID) panel to rapidly detect pathogens producing late-onset ventilator-associated pneumonia (VAP), a severe infection often produced by Gram-negative bacteria. These microorganisms are frequently multidrug resistant and typically require broad-spectrum empiric treatment. In the context of an international multicentre clinical trial (MagicBullet), respiratory samples were collected at the time of suspicion of VAP from 165 patients in 32 participating hospitals in Spain, Greece and Italy. Microorganisms were identified using the BCID panel and compared with results obtained by conventional microbiologic techniques. Pseudomonas aeruginosa, Acinetobacter baumannii and Klebsiella pneumoniae were the most commonly identified species, representing 54.7% (70/128) of microorganisms. The BCID panel showed high global specificity (98.1%; 95% confidence interval, 96–100) and negative predictive values (96.6%) and a global sensitivity and positive predictive value of 78.6% (95% confidence interval, 70–88) and 87.3%, respectively, for these microorganisms. Importantly, the BCID panel provided results in only 1 hour directly from respiratory samples with minimal sample processing times. The BCID panel may have clinical utility in rapidly ruling out microorganisms causing VAP, specifically multidrug-resistant Gram-negative species. This could facilitate the optimization of empiric treatment.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1198-743X 1469-0691
DOI:	10.1016/j.cmi.2018.06.001