Search Results - "Ramírez, Octavio T"

New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol by Martínez-Gómez, Karla, Flores, Noemí, Castañeda, Héctor M, Martínez-Batallar, Gabriel, Hernández-Chávez, Georgina, Ramírez, Octavio T, Gosset, Guillermo, Encarnación, Sergio, Bolivar, Francisco

“…Glycerol has enhanced its biotechnological importance since it is a byproduct of biodiesel synthesis. A study of Escherichia coli physiology during growth on…”
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Overexpression of the mitochondrial pyruvate carrier reduces lactate production and increases recombinant protein productivity in CHO cells by Bulté, Dubhe B., Palomares, Laura A., Parra, Carolina Gómez, Martínez, Juan Andrés, Contreras, Martha A., Noriega, Lilia G., Ramírez, Octavio T.

“…Chinese hamster ovary (CHO) cells are characterized by a low glucose catabolic efficiency, resulting in undesirable lactate production. Here, it is…”
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Expression in CHO cells of a bacterial biosynthetic pathway producing a small non-ribosomal peptide aldehyde prevents proteolysis of recombinant proteins by Guadarrama-Pérez, Violeta, Aguilar, César, Porras-Sanjuanico, Alberto, Merino, Enrique, Ramírez, Octavio T., Barona-Gómez, Francisco, Palomares, Laura A.

“…A significant problem during recombinant protein production is proteolysis. One of the most common preventive strategies is the addition of protease…”
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Production of recombinant proteins in E. coli by the heat inducible expression system based on the phage lambda pL and/or pR promoters by Valdez-Cruz, Norma A, Caspeta, Luis, Pérez, Néstor O, Ramírez, Octavio T, Trujillo-Roldán, Mauricio A

“…The temperature inducible expression system, based on the pL and/or pR phage lambda promoters regulated by the thermolabile cI857 repressor has been widely use…”
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Mimotope discovery as a tool to design a vaccine against Zika and dengue viruses by Cuevas‐Juárez, Esmeralda, Liñan‐Torres, Arturo, Hernández, Carolina, Kopylov, Mykhailo, Potter, Clint S., Carragher, Bridget, Ramírez, Octavio T., Palomares, Laura A.

“…Vaccine development against dengue virus is challenging because of the antibody‐dependent enhancement of infection (ADE), which causes severe disease…”
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Effect of sericin, a silk derived protein, on the amplification of Zika virus in insect and mammalian cell cultures by Alcalá, Ana C., Contreras, Martha A., Cuevas-Juárez, Esmeralda, Ramírez, Octavio T., Palomares, Laura A.

“…Sericin, a silk-derived non-immunogenic protein, has been used to improve cell culture performance by increasing viability, cell concentration, and promoting…”
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Xylose-glucose co-fermentation to ethanol by Escherichia coli strain MS04 using single- and two-stage continuous cultures under micro-aerated conditions by Fernández-Sandoval, Marco T, Galíndez-Mayer, Juvencio, Bolívar, Francisco, Gosset, Guillermo, Ramírez, Octavio T, Martinez, Alfredo

“…Simultaneous co-fermentation of mixed sugars is an important feature to consider in the production of ethanol from lignocellulosic biomass hydrolysates because…”
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A novel method for the in vitro assembly of virus-like particles and multimeric proteins by Rodríguez, Mabel, Castro-Acosta, Ricardo M., Ruiz-Morales, Elias R., Villanueva-Flores, Francisca, Ramírez, Octavio T., Palomares, Laura A.

“…Objective To develop a method for the efficient assembly of viral or multimeric proteins into virus-like particles (VLP) or other macro structures. Results…”
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Real-time imaging reveals unique heterogeneous population features in insect cell cultures by Hidalgo, David, Paz, Enrique, Palomares, Laura A., Ramírez, Octavio T.

“…•Live cell imaging revealed heterogeneous populations in Sf9 insect cell cultures.•Live cell imaging revealed the presence of motile elongated cells that were…”
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Physiological effects of pH gradients on Escherichia coli during plasmid DNA production by Cortés, José T., Flores, Noemí, Bolívar, Francisco, Lara, Alvaro R., Ramírez, Octavio T.

“…ABSTRACT A two‐compartment scale‐down system was used to mimic pH heterogeneities that can occur in large‐scale bioreactors. The system consisted of two…”
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Defining the multiplicity and time of infection for the production of Zaire Ebola virus-like particles in the insect cell-baculovirus expression system by Pastor, Ana Ruth, González-Domínguez, Gonzalo, Díaz-Salinas, Marco A., Ramírez, Octavio T., Palomares, Laura A.

“…The Ebola virus disease is a public health challenge. To date, the only available treatments are medical support or the emergency administration of…”
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Engineering Escherichia coli to increase plasmid DNA production in high cell-density cultivations in batch mode by Borja, Gheorghe M, Meza Mora, Eugenio, Barrón, Blanca, Gosset, Guillermo, Ramírez, Octavio T, Lara, Alvaro R

“…Plasmid DNA (pDNA) is a promising molecule for therapeutic applications. pDNA is produced by Escherichia coli in high cell-density cultivations (HCDC) using…”
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Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system by Escalante, Adelfo, Calderón, Rocío, Valdivia, Araceli, de Anda, Ramón, Hernández, Georgina, Ramírez, Octavio T, Gosset, Guillermo, Bolívar, Francisco

“…Shikimic acid (SA) is utilized in the synthesis of oseltamivir-phosphate, an anti-influenza drug. In this work, metabolic engineering approaches were employed…”
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Plasmid DNA production for therapeutic applications by Lara, Alvaro R, Ramírez, Octavio T, Wunderlich, Martin

“…Plasmid DNA (pDNA) is the base for promising DNA vaccines and gene therapies against many infectious, acquired, and genetic diseases, including HIV-AIDS,…”
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Effect of growth rate on plasmid DNA production and metabolic performance of engineered Escherichia coli strains by Wunderlich, Martin, Taymaz-Nikerel, Hilal, Gosset, Guillermo, Ramírez, Octavio T., Lara, Alvaro R.

“…Two engineered Escherichia coli strains, designated VH33 and VH34, were compared to their parent strain W3110 in chemostat mode during plasmid DNA (pDNA)…”
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effect of heating rate on Escherichia coli metabolism, physiological stress, transcriptional response, and production of temperature-induced recombinant protein: A scale-down study by Caspeta, Luis, Flores, Noemí, Pérez, Néstor O, Bolívar, Francisco, Ramírez, Octavio T

“…At the laboratory scale, sudden step increases from 30 to 42°C can be readily accomplished when expressing heterologous proteins in heat-inducible systems…”
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Fast dynamic response of the fermentative metabolism of Escherichia coli to aerobic and anaerobic glucose pulses by Lara, Alvaro R, Taymaz-Nikerel, Hilal, Mashego, Mlawule R, van Gulik, Walter M, Heijnen, Joseph J, Ramírez, Octavio T, van Winden, Wouter A

“…The response of Escherichia coli cells to transient exposure (step increase) in substrate concentration and anaerobiosis leading to mixed-acid fermentation…”
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Flocculation of CHO cells for primary separation of recombinant glycoproteins: Effect on glycosylation profiles by Gómez, Itzcóatl A., Hernández, Vanessa, Palomares, Laura A., Ramírez, Octavio T.

“…[Display omitted] •The effect of flocculation on the glycosylation profile of proteins was studied.•Residual DNA and host cell proteins were efficiently…”
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Living with heterogeneities in bioreactors: understanding the effects of environmental gradients on cells by Lara, Alvaro R, Galindo, Enrique, Ramírez, Octavio T, Palomares, Laura A

“…The presence of spatial gradients in fundamental culture parameters, such as dissolved gases, pH, concentration of substrates, and shear rate, among others, is…”
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The assembly conformation of rotavirus VP6 determines its protective efficacy against rotavirus challenge in mice by Pastor, Ana Ruth, Rodríguez-Limas, William A, Contreras, Martha A, Esquivel, Ernesto, Esquivel-Guadarrama, Fernando, Ramírez, Octavio T, Palomares, Laura A

“…Abstract Viral protein assemblies have shown to be superior immunogens used in commercial vaccines. However, little is known about the effect of protein…”
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