Effect of cold plasma on the E. coli cell wall and plasma membrane

Effect of cold plasma on the E. coli cell wall and plasma membrane

The effect of cold plasma on E. coli cells was studied. It was shown that the treatment of E. coli cells with cold plasma caused partial or total disruption of the plasma membrane integrity, which was accompanied by a release of intracellular substances into the extracellular environment. A quantita...

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Bibliographic Details
Published in:Applied biochemistry and microbiology Vol. 49; no. 2; pp. 144 - 149
Main Authors: Kobzev, E. N, Kireev, G. V, Rakitskii, Yu. A, Martovetskaya, I. I, Chugunov, V. A, Kholodenko, V. P, Khramov, M. V, Akishev, Yu. S, Trushkin, N. I, Grushin, M. E
Format: Journal Article
Language:English
Published: Dordrecht Springer-Verlag 01-03-2013
SP MAIK Nauka/Interperiodica
Springer Nature B.V
Subjects:
Biochemistry
Biomedical and Life Sciences
cell death
Cell Membrane - drug effects
Cell Membrane - metabolism
Cell Membrane Permeability - drug effects
Cell Wall - drug effects
Cell Wall - metabolism
cell walls
Culture Media
E coli
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - growth & development
Escherichia coli - metabolism
ionic strength
Life Sciences
Medical Microbiology
Membranes
Microbiology
Nucleotides - metabolism
Osmolar Concentration
Osmotic Pressure
osmotic stress
Plasma
Plasma Gases - pharmacology
plasma membrane
Temperature effects
Plasma Membrane Integrity
Osmotic Pressure
Plasma Treatment
Cold Plasma
Apply Biochemistry
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Summary:The effect of cold plasma on E. coli cells was studied. It was shown that the treatment of E. coli cells with cold plasma caused partial or total disruption of the plasma membrane integrity, which was accompanied by a release of intracellular substances into the extracellular environment. A quantitative assessment of the extent of the damage to the cell membrane showed that a loss of no more than 23.6% of intracellular substances (calculated by the proportion of the intracellular nucleotide release) is sufficient to lead to cell death. The use of media with different ionic strength levels to create osmotic shock showed that the treatment of E. coli cells with cold plasma significantly decreased the cell wall strength.
Bibliography:http://dx.doi.org/10.1134/S0003683813020063
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-6838
0555-1099
1608-3024
DOI:10.1134/s0003683813020063