Search Results - "Rajagopalan, K V"

Mechanism of ubiquitin activation revealed by the structure of a bacterial MoeB-MoaD complex by Schindelin, Hermann, Lake, Michael W, Wuebbens, Margot M, Rajagopalan, K. V

“…The activation of ubiquitin and related protein modifiers is catalysed by members of the E1 enzyme family that use ATP for the covalent self-attachment of the…”
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Layer-by-Layer Arrangement by Protein−Protein Interaction of Sulfite Oxidase and Cytochrome c Catalyzing Oxidation of Sulfite by Dronov, Roman, Kurth, Dirk G, Möhwald, Helmuth, Spricigo, Roberto, Leimkühler, Silke, Wollenberger, Ulla, Rajagopalan, K. V, Scheller, Frieder W, Lisdat, Fred

“…Layer-by-layer self-assembly of sulfite oxidase and cytochrome c was carried out without additional polymeric polyelectrolytes. The arrangement shows a linear…”
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Effect of Exchange of the Cysteine Molybdenum Ligand with Selenocysteine on the Structure and Function of the Active Site in Human Sulfite Oxidase by Reschke, Stefan, Niks, Dimitri, Wilson, Heather, Sigfridsson, Kajsa G. V, Haumann, Michael, Rajagopalan, K. V, Hille, Russ, Leimkühler, Silke

“…Sulfite oxidase (SO) is an essential molybdoenzyme for humans, catalyzing the final step in the degradation of sulfur-containing amino acids and lipids, which…”
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Structure-Based Alteration of Substrate Specificity and Catalytic Activity of Sulfite Oxidase from Sulfite Oxidation to Nitrate Reduction by Qiu, James A, Wilson, Heather L, Rajagopalan, K. V

“…Eukaryotic sulfite oxidase is a dimeric protein that contains the molybdenum cofactor and catalyzes the metabolically essential conversion of sulfite to…”
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Structure of the Molybdenum Site of Escherichia coli Trimethylamine N-Oxide Reductase by Zhang, Limei, Nelson, Kimberly Johnson, Rajagopalan, K. V, George, Graham N

“…We report a structural characterization of the molybdenum site of recombinant Escherichia coli trimethylamine N-oxide (TMAO) reductase using X-ray absorption…”
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Evidence for the Physiological Role of a Rhodanese-Like Protein for the Biosynthesis of the Molybdenum Cofactor in Humans by Matthies, Andreas, Rajagopalan, K. V., Mendel, Ralf R., Leimkühler, Silke, Matthews, Rowena G.

“…Recent studies have identified the human genes involved in the biosynthesis of the molybdenum cofactor. The human MOCS3 protein contains an N-terminal domain…”
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Crystal Structure of DMSO Reductase: Redox-Linked Changes in Molybdopterin Coordination by Schindelin, Hermann, Kisker, Caroline, Hilton, James, Rajagopalan, K. V., Rees, Douglas C.

“…The molybdoenzyme dimethylsulfoxide (DMSO) reductase contributes to the release of dimethylsulfide, a compound that has been implicated in cloud nucleation and…”
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Crystal Structure of a Molybdopterin Synthase−Precursor Z Complex:  Insight into Its Sulfur Transfer Mechanism and Its Role in Molybdenum Cofactor Deficiency by Daniels, Juma N, Wuebbens, Margot M, Rajagopalan, K. V, Schindelin, Hermann

“…In almost all biological life forms, molybdenum and tungsten are coordinated by molybdopterin (MPT), a tricyclic pyranopterin containing a cis-dithiolene…”
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In Vitro Molybdenum Ligation to Molybdopterin Using Purified Components by Nichols, Jason D, Rajagopalan, K V

“…We have previously shown that Escherichia coli MoeA and MogA are required in vivo for the final step of molybdenum cofactor biosynthesis, the addition of the…”
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Structural Insights into Sulfite Oxidase Deficiency by Karakas, Erkan, Wilson, Heather L., Graf, Tyler N., Xiang, Song, Jaramillo-Busquets, Sandra, Rajagopalan, K.V., Kisker, Caroline

“…Sulfite oxidase deficiency is a lethal genetic disease that results from defects either in the genes encoding proteins involved in molybdenum cofactor…”
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The Structures of the C185S and C185A Mutants of Sulfite Oxidase Reveal Rearrangement of the Active Site by Qiu, James A, Wilson, Heather L, Pushie, M. Jake, Kisker, Caroline, George, Graham N, Rajagopalan, K. V

“…Sulfite oxidase (SO) catalyzes the physiologically critical conversion of sulfite to sulfate. Enzymatic activity is dependent on the presence of the metal…”
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Mechanistic and Mutational Studies of Escherichia coli Molybdopterin Synthase Clarify the Final Step of Molybdopterin Biosynthesis by Wuebbens, Margot M., Rajagopalan, K.V.

“…Biosynthesis of the molybdenum cofactor, a chelate of molybdenum or tungsten with a novel pterin, occurs in virtually all organisms including humans. In the…”
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A Sulfurtransferase Is Required in the Transfer of Cysteine Sulfur in the in Vitro Synthesis of Molybdopterin from Precursor Z in Escherichia coli by Leimkühler, Silke, Rajagopalan, K.V.

“…It has been shown that conversion of precursor Z to molybdopterin (MPT) by Escherichia coli MPT synthase entails the transfer of the sulfur atom of the…”
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The Role of Tyrosine 343 in Substrate Binding and Catalysis by Human Sulfite Oxidase by Wilson, Heather L., Rajagopalan, K.V.

“…In the crystal structure of chicken sulfite oxidase, the residue Tyr322 (Tyr343 in human sulfite oxidase) was found to directly interact with a bound sulfate…”
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Characterization of Escherichia coli MoeB and Its Involvement in the Activation of Molybdopterin Synthase for the Biosynthesis of the Molybdenum Cofactor by Leimkühler, Silke, Wuebbens, Margot M., Rajagopalan, K.V.

“…Amino acid sequence comparisons ofEscherichia coli MoeB suggested that the MoeB-dependent formation of a C-terminal thiocarboxylate on the MoaD subunit of…”
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Structure of the Molybdenum Site of Dimethyl Sulfoxide Reductase by George, Graham N, Hilton, James, Temple, Carrie, Prince, Roger C, Rajagopalan, K. V

“…Molybdenum K-edge X-ray absorption and Mo(V) electron paramagentic resonance (EPR) spectroscopies have been used to probe the metal coordination in oxidized…”
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Role of the C-Terminal Gly-Gly Motif of Escherichia Coli MoaD, a Molybdenum Cofactor Biosynthesis Protein with a Ubiquitin Fold by Schmitz, Jennifer, Wuebbens, Margot M, Rajagopalan, K. V, Leimkühler, Silke

“…In Escherichia coli, the MoaD protein plays a central role in the conversion of precursor Z to molybdopterin (MPT) during molybdenum cofactor biosynthesis…”
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Nature of Halide Binding to the Molybdenum Site of Sulfite Oxidase by Pushie, M. Jake, Doonan, Christian J, Wilson, Heather L, Rajagopalan, K. V, George, Graham. N

“…Valuable information on the active sites of molybdenum enzymes has been provided from both MoV electron paramagnetic resonance (EPR) spectroscopy and X-ray…”
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Modified Active Site Coordination in a Clinical Mutant of Sulfite Oxidase by Doonan, Christian J, Wilson, Heather L, Rajagopalan, K. V, Garrett, Robert M, Bennett, Brian, Prince, Roger C, George, Graham N

“…The molybdenum site of the Arginine 160 → Glutamine clinical mutant of the physiologically vital enzyme sulfite oxidase has been investigated by a combination…”
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High-Resolution EXAFS of the Active Site of Human Sulfite Oxidase:  Comparison with Density Functional Theory and X-ray Crystallographic Results by Harris, Hugh H, George, Graham N, Rajagopalan, K. V

“…Much of our knowledge about molybdenum enzymes has originated from EXAFS spectroscopy. This technique provides excellent bond-length accuracy but has only…”
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