Search Results - "Raia, C. A"

Asn249Tyr Substitution at the Coenzyme Binding Domain Activates Sulfolobus solfataricus Alcohol Dehydrogenase and Increases Its Thermal Stability by Giordano, A, Cannio, R, La Cara, F, Bartolucci, S, Rossi, M, Raia, C. A

“…A mutant of the thermostable NAD+-dependent homotetrameric alcohol dehydrogenase from Sulfolobus solfataricus (SsADH), which has a single substitution,…”
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Alcohol dehydrogenase from Sulfolobus solfataricus by Raia, C A, Giordano, A, Rossi, M

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Malic enzyme from archaebacterium Sulfolobus solfataricus. Purification, structure, and kinetic properties by Bartolucci, S, Rella, R, Guagliardi, A, Raia, C A, Gambacorta, A, De Rosa, M, Rossi, M

“…An NADP-preferring malic enzyme ((S)-malate:NADP oxidoreductase (oxalacetate-decarboxylating) EC 1.1.1.40) with a specific activity of 36.6 units per mg of…”
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Thermostable β‐galactosidase from the archaebacterium Sulfolobus solfataricus Purification and properties by PISANI, Francesca M., RELLA, Rocco, RAIA, Carlo A., ROZZO, Carla, NUCCI, Roberto, GAMBACORTA, Agata, ROSA, Mario, ROSSI, Mosè

“…A thermophilic and thermostable β‐galactosidase activity was purified to homogeneity from crude extracts of the archaebacterium Sulfolobus solfataricus, by a…”
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Protein Crystal Growth in the Advanced Protein Crystallization Facility on the LMS Mission: a Comparison of Sulfolobus solfataricus Alcohol Dehydrogenase Crystals Grown on the Ground and in Microgravity by Esposito, L., Sica, F., Sorrentino, G., Berisio, R., Carotenuto, L., Giordano, A., Raia, C. A., Rossi, M., Lamzin, V. S., Wilson, K. S., Zagari, A.

“…Crystals of alcohol dehydrogenase from Sulfolobus solfataricus were grown in the Advanced Protein Crystallization Facility during the Life and Microgravity…”
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Allosteric modifier and substrate binding of donkey deoxycytidylate aminohydrolase (EC 3.5.4.12) by Nucci, R, Raia, C A, Vaccaro, C, Rossi, M, Whitehead, E P

“…The hexameric allosteric enzyme deoxycytidylate aminohydrolase from donkey spleen is shown by equilibrium dialysis to bind specifically the allosteric…”
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Purification and characterization of a thermostable carboxypeptidase from the extreme thermophilic archaebacterium Sulfolobus solfataricus by COLOMBO, Sonia, D'AURIA, Sabato, FUSI, Paola, ZECCA, Laura, RAIA, Carlo Antonio, TORTORA, Paolo

“…A carboxypeptidase was purified to electrophoretic homogeneity from the thermoacidophilic archaebacterium Sulfolobus solfataricus. Molecular masses assessed by…”
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Thermostable NAD(+)-dependent alcohol dehydrogenase from Sulfolobus solfataricus: gene and protein sequence determination and relationship to other alcohol dehydrogenases by Ammendola, S, Raia, C A, Caruso, C, Camardella, L, D'Auria, S, De Rosa, M, Rossi, M

“…The NAD(+)-dependent alcohol dehydrogenase (EC 1.1.1.1) from the thermoacidophilic archaebacterium Sulfolobus solfataricus, DSM1617 strain (SSADH), has been…”
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Chemical stabilization of conformational states of dCMP deaminase by Rossi, M, Raia, C A, Vaccaro, C

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A novel archaebacterial NAD+-dependent alcohol dehydrogenase: purification and properties by RELLA, R, RAIA, C. A, PENSA, M, PISANI, F. M, GAMBACORTA, A, DE ROSA, M, ROSSI, M

“…An NAD+-dependent alcohol dehydrogenase (alcohol: NAD+ oxidoreductase, EC 1.1.1.1) was detected in cellular extracts of the extreme thermophilic…”
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Reversal of the effect of the allosteric ligands of dCMP-aminohydrolase and stabilization of the enzyme in the T form by Raia, C A, Nucci, R, Vaccaro, C, Sepe, S, Rella, R, Rossi, M

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Purification and properties of a thermophilic and thermostable DNA polymerase from the archaebacterium Sulfolobus solfataricus by Rella, R, Raia, C A, Pisani, F M, D'Auria, S, Nucci, R, Gambacorta, A, De Rosa, M, Rossi, M

“…A DNA-dependent DNA polymerase was obtained in homogenous form from the thermoacidophilic archaebacterium Sulfolobus solfataricus. The enzyme, purified…”
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Activation of Sulfolobus solfataricus Alcohol Dehydrogenase by Modification of Cysteine Residue 38 with Iodoacetic Acid by Raia, Carlo A, Caruso, Carla, Marino, Monica, Vespa, Nunzia, Rossi, Mosè

“…Reaction of thermostable NAD+-dependent alcohol dehydrogenase from Sulfolobus solfataricus with iodoacetate at pH 9.0 and 37 °C significantly increases the…”
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Crystallization and preliminary X-ray analysis of an NAD(+)-dependent alcohol dehydrogenase from the extreme thermophilic archaebacterium Sulfolobus solfataricus by Pearl, L H, Demasi, D, Hemmings, A M, Sica, F, Mazzarella, L, Raia, C A, D'Auria, S, Rossi, M

“…An NAD(+)-dependent alcohol dehydrogenase from the extreme thermophilic archaebacterium Sulfolobus solfataricus has been crystallized in the holo-enzyme and…”
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5-AZA-2'-deoxycytidine synergistic action with thymidine on leukemic cells and interaction of 5-AZA-dCMP with dCMP deaminase by Momparler, R L, Rossi, M, Bouchard, J, Bartolucci, S, Momparler, L F, Raia, C A, Nucci, R, Vaccaro, C, Sepe, S

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Determination of hydride transfer stereospecificity of NADH-dependent alcohol-aldehyde/ketone oxidoreductase from Sulfolobus solfataricus by Trincone, A, Lama, L, Rella, R, D'Auria, S, Raia, C A, Nicolaus, B

“…This paper describes the determination of stereospecificity of hydride transfer reaction of an alcohol dehydrogenase isolated from the archaebacterium…”
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Freezing of dCMP aminohydrolase in the activated conformation by glutaraldehyde by Nucci, R, Rala, C A, Vaccaro, C, Sepe, S, Scarano, E, Rossi, M

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Crystal Structure of a Ternary Complex of the Alcohol Dehydrogenase from Sulfolobus solfataricus by Esposito, Luciana, Bruno, Ilaria, Sica, Filomena, Raia, Carlo Antonio, Giordano, Antonietta, Rossi, Mosè, Mazzarella, Lelio, Zagari, Adriana

“…The crystal structure of a ternary complex of the alcohol dehydrogenase from the archaeon Sulfolobus solfataricus (SsADH) has been determined at 2.3 Å. The…”
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Evidence for co-operativity in coenzyme binding to tetrameric Sulfolobus solfataricus alcohol dehydrogenase and its structural basis: fluorescence, kinetic and structural studies of the wild-type enzyme and non-co-operative N249Y mutant by Giordano, Antonietta, Febbraio, Ferdinando, Russo, Consiglia, Rossi, Mosè, Raia, Carlo A

“…The interaction of coenzyme with thermostable homotetrameric NAD(H)-dependent alcohol dehydrogenase from the thermoacidophilic sulphur-dependent crenarchaeon…”
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Temperature-Induced Conformational Change at the Catalytic Site of Sulfolobus solfataricus Alcohol Dehydrogenase Highlighted by Asn249Tyr Substitution. A Hydrogen/Deuterium Exchange, Kinetic, and Fluorescence Quenching Study by Secundo, Francesco, Russo, Consiglia, Giordano, Antonietta, Carrea, Giacomo, Rossi, Mosè, Raia, Carlo A

“…A combination of hydrogen/deuterium exchange, fluorescence quenching, and kinetic studies was used to acquire experimental evidence for the…”
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