SCAR/WAVE complex recruitment to a supracellular actomyosin cable by myosin activators and a junctional Arf-GEF during Drosophila dorsal closure

Expansive Arp2/3 actin networks and contractile actomyosin networks can be spatially and temporally segregated within the cell, but the networks also interact closely at various sites, including adherens junctions. However, molecular mechanisms coordinating these interactions remain unclear. We foun...

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Bibliographic Details
Published in:	Molecular biology of the cell Vol. 33; no. 8; p. br12
Main Authors:	Hunt, Erin L, Rai, Hrishika, Harris, Tony J C
Format:	Journal Article
Language:	English
Published:	United States American Society for Cell Biology 01-07-2022 The American Society for Cell Biology
Subjects:	Actins - metabolism Actomyosin - metabolism Adherens Junctions - metabolism Analysis Animals Brief Reports Drosophila Drosophila - metabolism Drosophila melanogaster - metabolism Drosophila Proteins - metabolism Embryonic development G proteins Growth Identification and classification Mammals - metabolism Methods Muscle proteins Myosin Myosins - metabolism Properties India
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Summary:	Expansive Arp2/3 actin networks and contractile actomyosin networks can be spatially and temporally segregated within the cell, but the networks also interact closely at various sites, including adherens junctions. However, molecular mechanisms coordinating these interactions remain unclear. We found that the SCAR/WAVE complex, an Arp2/3 activator, is enriched at adherens junctions of the leading edge actomyosin cable during dorsal closure. Myosin activators were both necessary and sufficient for SCAR/WAVE accumulation at leading edge junctions. The same myosin activators were previously shown to recruit the cytohesin Arf-GEF Steppke to these sites, and mammalian studies have linked Arf small G protein signaling to SCAR/WAVE activation. During dorsal closure, we find that Steppke is required for SCAR/WAVE enrichment at the actomyosin-linked junctions. Arp2/3 also localizes to adherens junctions of the leading edge cable. We propose that junctional actomyosin activity acts through Steppke to recruit SCAR/WAVE and Arp2/3 for regulation of the leading edge supracellular actomyosin cable during dorsal closure.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1059-1524 1939-4586
DOI:	10.1091/mbc.E22-03-0107