The non‐ionizing electromagnetic stimulation enhanced antibody production (NESEAP) effect – Discovery and technological applications
The rise of biological therapeutics in the global pharmaceuticals market has escalated the demand for quality monoclonal antibodies for healthcare and scientific applications. Reducing costs while enhancing production yields without compromising quality are the main challenges to the growth of this...
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| Published in: | Biotechnology journal Vol. 19; no. 1; pp. e2300277 - n/a |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Germany
01-01-2024
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | The rise of biological therapeutics in the global pharmaceuticals market has escalated the demand for quality monoclonal antibodies for healthcare and scientific applications. Reducing costs while enhancing production yields without compromising quality are the main challenges to the growth of this industry today. Over the last two decades non‐ionizing radiation has been demonstrated to elicit targeted biological responses in a frequency and dose dependent manner. We hypothesize and design a millimeter wave radiation procedure to enhance the yields of antibody‐producing hybridoma cell lines. We demonstrate this method enhances the production of IgA and IgG antibodies from MOPC315.BM and U13.6 cells by a factor of 24.05 ± 3.32 and 1.41 ± 0.03 respectively relative to untreated cells. No treatment associated cytotoxicity was observed in either cell line corroborating physiological viability of irradiated cells. Our results demonstrate proof‐of‐concept of a novel technique to significantly enhance antibody yields from hybridoma cells which could lead to a reduction in antibody production costs. Further studies will focus on scaling up of this technology and employment of non‐contact, tuned electromagnetic stimulation of biological systems for targeted responses.
Graphical and Lay Summary
Many studies have investigated and reported that non‐ionizing millimeter waves (MMWs) induce a variety of biological effects which are power dose dependent with reversible non‐thermal effects being achieved only when dose is within the International Commission on Non‐Ionizing Radiation Protection (ICNIRP) exposure safety stipulations. However, no publication till date investigated whether low dose MMW exposure could specifically induce increased production of antibodies. As proof‐of‐principle we devised and tested an in vitro setup and treatment regime operating within the safe MMW exposure regimes and demonstrated that murine hybridoma cells increase their mAb yields after electromagnetic stimulation with no reduction in cell viabilities. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1860-6768 1860-7314 |
| DOI: | 10.1002/biot.202300277 |