Search Results - "Raftos, J E"

Effect of intracellular magnesium on calcium extrusion by the plasma membrane calcium pump of intact human red cells by Raftos, J E, Lew, V L

“…1. The effect of varying the concentration of intracellular magnesium on the Ca(2+)-saturated Ca(2+)-extrusion rate through the Ca2+ pump (phi max) was…”
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Distribution of chloride permeabilities in normal human red cells by Raftos, J E, Bookchin, R M, Lew, V L

“…1. The rate of dehydration of K+ permeabilized red cells is influenced by their Cl- permeability (PCl). In instances of pathological K+ permeabilization,…”
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Measurement of the distribution of anion exchange function in normal human red cells by Raftos, J E, Bookchin, R M, Lew, V L

“…1. The aim of the present work was to investigate cell-to-cell variation in anion exchange turnover in normal human red cells. Red cells permeabilized to…”
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Generation of Normal Human Red Cell Volume, Hemoglobin Content, and Membrane Area Distributions by “Birth” or Regulation? by Lew, Virgilio L., Raftos, Julia E., Sorette, Martin, Bookchin, Robert M., Mohandas, Narla

“…Using flow cytometry and osmotic lysis measurements, we document here the means and coefficients of variation of the following red cell (RBC) properties:…”
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Normal Ca2+ extrusion by the Ca2+ pump of intact red blood cells exposed to high glucose concentrations by Raftos, J E, Edgley, A, Bookchin, R M, Etzion, Z, Lew, V L, Tiffert, T

“…The ATPase activity of the plasma membrane Ca2+ pump (PMCA) has been reported to be inhibited by exposure of red blood cell (RBC) PMCA preparations to high…”
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Regeneration of phosphorylated metabolites in stored erythrocytes in an open perfusion system: studies using 31P NMR spectroscopy by Chapman, B E, Kuchel, P W, Lovric, V A, Raftos, J E, Stewart, I M

“…Human erythrocytes were maintained at high haematocrit in a metabolically functional state for several hours in a thermodynamically open perfusion apparatus…”
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The relationship between glucose concentration and rate of lactate production by human erythrocytes in an open perfusion system by Kuchel, P W, Chapman, B E, Lovric, V A, Raftos, J E, Stewart, I M, Thorburn, D R

“…A thermodynamically open system, based on an assembly of capillaries with semi-permeable walls was constructed in order to study glycolysis in human…”
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Red cells suspended in a phosphate-fortified additive: biochemical enhancement and longer storage by Lovric, V A, Carr, G, Raftos, J E, Stewart, I M, Trueman, K

“…When compared to our current storage regime, an additive solution, with 75 mM inorganic phosphate, improved certain biochemical and morphological parameters…”
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Refinement and evaluation of a model of Mg2+ buffering in human red cells by Raftos, Julia E., Lew, Virgilio L., Flatman, Peter W.

“…The total Mg2+ content of human red cells ([Mg]T,i) is partitioned between free and bound forms. The main cytoplasmic Mg2+ buffers are ATP and 2,3…”
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Evaluation of an electrochemical model of erythrocyte pH buffering using 31P nuclear magnetic resonance data by RAFTOS, J. E, BULLIMAN, B. T, KUCHEL, P. W

“…When erythrocytes are suspended in a solution of known composition the resultant values of such basic cell parameters as volume and pH are difficult to…”
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Further investigation of the use of dimethyl methylphosphonate as a 31P-NMR probe of red cell volume by Raftos, J E, Kirk, K, Kuchel, P W

“…We have refined a method for measuring erythrocyte volume using the 31P-NMR spectrum of a probe molecule, dimethyl methylphosphonate. This compound, when added…”
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Intra- and extraerythrocyte pH at 37 degrees C and during long term storage at 4 degrees C: 31P NMR measurements and an electrochemical model of the system by Raftos, J E, Chapman, B E, Kuchel, P W, Lovric, V A, Stewart, I M

“…A 31P NMR method based on pH dependent variation of the chemical-shift-difference between the resonances of orthophosphate and methylphosphonate was used to…”
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Intracellular pH in stored erythrocytes. Refinement and further characterisation of the 31P-NMR methylphosphonate procedure by Stewart, I M, Chapman, B E, Kirk, K, Kuchel, P W, Lovric, V A, Raftos, J E

“…Methylphosphonate in conjunction with 31P-NMR spectroscopy was used for the measurement of transmembrane delta pH in human erythrocytes stored at 4 degrees C…”
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Supernatant hemoglobin determinations after prolonged blood storage by Raftos, J E, Stewart, I M, Lovric, V A

“…Measurement of extracellular hemoglobin is useful for assessing the relative success of different blood storage strategies. The test is now also included in…”
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Generation of normal human red cell volume, hemoglobin content, and membrane area distribution by birth or regulation ? by LEW, V. L, RAFTOS, J. E, SORETTE, M, BOOKCHIN, R. M, MOHANDAS, N

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Direct measurement of the rate of glutathione synthesis in 1-chloro-2,4-dinitrobenzene treated human erythrocytes by Raftos, Julia E, Dwarte, Tanya M, Luty, Agnes, Willcock, Christopher J

“…Cell glutathione scavenges free radicals, degrades peroxides, removes damaging electrophiles and maintains the redox state. The aim of this study was to…”
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