Search Results - "Raf, Lemmens"

A chromatographic method for determination of supercoiled plasmid DNA concentration in complex solutions by Bennemo, Mia, Blom, Hans, Emilsson, Anna, Lemmens, Raf

“…A method for determination of the plasmid DNA concentration with subsequent analysis of the ratio supercoiled to open circular form is presented. The method is…”
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Supercoiled plasmid DNA: selective purification by thiophilic/aromatic adsorption by Lemmens, Raf, Olsson, Urban, Nyhammar, Tomas, Stadler, Joachim

“…Separation of the different plasmid isoforms is a major challenge in purifying plasmid DNA. We describe a new type of biochemical interaction that occurs in…”
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Thiophilic interaction chromatography for supercoiled plasmid DNA purification by Sandberg, Lena M, Bjurling, Åsa, Busson, Philippe, Vasi, Jozsef, Lemmens, Raf

“…Supercoiled plasmid DNA was selectively purified from its open circular form by thiophilic interaction chromatography, performed in the presence of high…”
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Identification and Characterization of a Novel Hepatic Canalicular ATP Diphosphohydrolase by Sévigny, Jean, Robson, Simon C., Waelkens, Etienne, Csizmadia, Eva, Smith, R.Neal, Lemmens, Raf

“…We have identified and characterized a novel ATP diphosphohydrolase (ATPDase) with features of E-type ATPases from porcine liver. Immunoblotting with a…”
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Plasmid DNA purification by Stadler, Joachim, Lemmens, Raf, Nyhammar, Tomas

“…The demand for efficient production methods of plasmid DNA (pDNA) has increased vastly in response to rapid advances in the use of pDNA in gene therapy and in…”
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Flocculate removal after alkaline lysis in plasmid DNA production by Blom, Hans, Bennemo, Mia, Berg, Mikael, Lemmens, Raf

“…Abstract Alkaline lysis is the most commonly used method following harvest of bacterial cells for production of plasmid DNA. The method was originally…”
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Extraction of plasmid DNA from Escherichia coli cell lysate in a thermoseparating aqueous two-phase system by Kepka, Cecilia, Rhodin, Jenny, Lemmens, Raf, Tjerneld, Folke, Gustavsson, Per-Erik

“…The primary purification of a 6.1 kilo base pair (kbp) plasmid from a desalted alkaline lysate has been accomplished by a thermoseparating aqueous two-phase…”
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Integrated process for purification of plasmid DNA using aqueous two-phase systems combined with membrane filtration and lid bead chromatography by Kepka, Cecilia, Lemmens, Raf, Vasi, Jozsef, Nyhammar, Tomas, Gustavsson, Per-Erik

“…An integrated process for purifying a 6.1 kilo base pair (kbp) plasmid from a clarified Escherichia coli cell lysate based on an ultra/diafiltration step…”
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Ca2+-induced Ca2+ Release from the Endoplasmic Reticulum Amplifies the Ca2+ Signal Mediated by Activation of Voltage-gated L-type Ca2+ Channels in Pancreatic β-Cells by Lemmens, Raf, Larsson, Olof, Berggren, Per-Olof, Islam, Md. Shahidul

“…Stimulus-secretion coupling in pancreatic β-cells involves membrane depolarization and Ca2+entry through voltage-gated L-type Ca2+ channels, which is one…”
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Purification of plasmid DNA with a new type of anion-exchange beads having a non-charged surface by Gustavsson, Per-Erik, Lemmens, Raf, Nyhammar, Tomas, Busson, Philippe, Larsson, Per-Olof

“…We have prepared a new type of anion exchanger, which effectively discriminates between RNA and plasmid DNA. The material is based on a Sephacryl S-500 HR…”
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Ryanodine receptors of pancreatic β‐cells mediate a distinct context‐dependent signal for insulin secretion by Bruton, Joseph D., Lemmens, Raf, Shi, Chun‐Liang, Persson‐Sjögren, Solveig, Westerblad, Håkan, Ahmed, Masroor, Pyne, Nigel J., Frame, Mhairi, Furman, Brian L., Islam, Md. Shahidul

“…The ryanodine (RY) receptors in β‐cells amplify signals by Ca2+‐induced Ca2+ release (CICR). The role of CICR in insulin secretion remains unclear in spite of…”
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173. Process Design and Scale-Up of New Vaccine Concepts for Biodefense by Herzer, Sibylle U, Bergvall, Peder, Lemmens, Raf, Coffey, Matt, Leps, Walter T, Moore, Peter M

“…New vaccine delivery constituents such as oligonucleotides, plasmids and infectious non-replicating viruses have created the need to find ways to purify these…”
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174. Process Development of a Scaleable Purification Protocol for the Production of Reolysin, an Oncolytic, Reovirus-Based Therapeutic by Herzer, Sibylle U, Peder, Bergvall, Raf, Lemmens, Matt, Coffey, Peter, Moore M

“…Reovirus is an enterovirus that specifically infects cells with an activated ras-pathway. As the activation of the ras-pathway is a common denominator in a…”
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Ca2+-induced Ca2+ Release from the Endoplasmic Reticulum Amplifies the Ca2+ Signal Mediated by Activation of Voltage-gated L-type Ca2+ Channels in Pancreatic β-Cells by Raf Lemmens, Olof Larsson, Per-Olof Berggren, Md. Shahidul Islam

“…Stimulus-secretion coupling in pancreatic β-cells involves membrane depolarization and Ca 2+ entry through voltage-gated L-type Ca 2+ channels, which is one…”
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Regulation of proliferation of LLC-MK2 cells by nucleosides and nucleotides: the role of ecto-enzymes by Lemmens, R, Vanduffel, L, Teuchy, H, Culic, O

“…1. Using the incorporation of [methyl-3H]thymidine as a proliferation marker, the effects of various nucleosides and nucleotides on endothelial LLC-MK2 cells…”
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Purification, characterization, and localization of an ATP diphosphohydrolase in porcine kidney by Lemmens, R, Kupers, L, Sévigny, J, Beaudoin, A R, Grondin, G, Kittel, A, Waelkens, E, Vanduffel, L

“…Membranes of pig kidney cortex tissue were solubilized in the presence of Triton X-100. Partial purification of ATP diphosphohydrolase (ATPDase) was achieved…”
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Study of ATP diphosphohydrolases in porcine kidney and liver by Lemmens, Raf

“…In this thesis, we describe the results of our studies on porcine ATP diphosphohydrolases. First, an ATPDase present in porcine kidney cortex was partially…”
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Distribution, cloning, and characterization of porcine nucleoside triphosphate diphosphohydrolase‐1 by Lemmens, Raf, Vanduffel, Luc, Kittel, Agnes, Beaudoin, Adrien R., Benrezzak, Ouhida, Sévigny, Jean

“…In this study, we have investigated the distribution of the enzyme nucleoside triphosphate diphosphohydrolase‐1 (NTPDase1; EC 3.6.1.5) in a subset of pig…”
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Autoradiography-based cytochemical detection of ecto-ATPase, ecto-ADPase, 5'-nucleotidase, and extracellular adenosine production, employing 141Ce3+ as a capturing agent by Culic, O, Lemmens, R, Teuchy, H, Vanduffel, L

“…A method for the visualization of the ecto-nucleotidase enzyme activities present on the cell surface, employing 141Ce3+ as a capturing and labelling agent, is…”
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Identification and Partial Purification of (Ca2+ or Mg2+)‐ATPase in Renal Brush‐Border Membranes by Erum, Monique, Lemmens, Raf, Berden, Jan, Teuchy, Henri, Vanduffel, Luc

“…The protein responsible for the (Ca2+ or Mg2+)‐ATPase activity in brush‐border membranes from pig kidney tubular cells was characterized to distinguish this…”
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