Phenotypic and Genotypic Screening of Colistin Resistance Associated with Emerging Pathogenic Escherichia coli Isolated from Poultry

Phenotypic and Genotypic Screening of Colistin Resistance Associated with Emerging Pathogenic Escherichia coli Isolated from Poultry

Chickens continue to be an important reservoir of zoonotic multidrug-resistant illnesses. Antimicrobial resistance correlated with colistin has emerged as a critical concern worldwide in the veterinary field and the public health sector. The current study investigated the prevalence of multidrug-res...

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Bibliographic Details
Published in:Veterinary sciences Vol. 9; no. 6; p. 282
Main Authors: Badr, Heba, Samir, Abdelhafez, El-Tokhi, Essam Ismail, Shahein, Momtaz A., Rady, Flourage M., Hakim, Ashraf S., Fouad, Ehab Ali, El-Sady, Engy Farahat, Ali, Samah F.
Format: Journal Article
Language:English
Published: Basel MDPI AG 09-06-2022
MDPI
Subjects:
Amino acids
Ampicillin
Antibiotics
Antimicrobial agents
Antimicrobial resistance
APEC
Bacteria
Clindamycin
Colistin
colistin resistance
Drug resistance
E coli
Egypt
Escherichia coli
Farms
Gram-negative bacteria
Laboratories
mcr-1
Minimum inhibitory concentration
Multidrug resistance
Pathogens
Peptides
Poultry
Public health
Serotypes
Streptomycin
United Kingdom > UK
Japan
Germany
Egypt
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Summary:Chickens continue to be an important reservoir of zoonotic multidrug-resistant illnesses. Antimicrobial resistance correlated with colistin has emerged as a critical concern worldwide in the veterinary field and the public health sector. The current study investigated the prevalence of multidrug-resistant avian pathogenic Escherichia coli among chicken farms in three Egyptian governorates, focusing on colistin resistance assessment. A total of 56 Escherichia coli isolates were recovered out of 120 pooled samples obtained from diseased chicken broilers (46.7%). The E. coli isolates were serotyped to nine different serotypes; the highest incidence was for O125 (n = 18). The E. coli isolates demonstrated multidrug-resistant patterns against 10 antibiotics, especially clindamycin, tetracycline, streptomycin and ampicillin, by 100, 100, 96.4 and 92.9%, respectively. On the other hand, colistin resistance was 41.1% using AST. All E. coli isolates displayed positive colistin resistance growth on chromogenic medium, but only 25% represented this positivity via MIC estimation and Sensititre kit. PCR results revealed that all isolates harbored mcr-1, but no isolates harbored the other 2–5 mcr genes. In conclusion, the study demonstrated the emergence of multidrug-resistant, especially colistin-resistant, E. coli among chicken broiler flocks, and mcr-1 is the master gene of the colistin resistance feature.
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ISSN:2306-7381
2306-7381
DOI:10.3390/vetsci9060282