Toxinological and immunological studies of capillary electrophoresis fractionated Chrysaora quinquecirrha (Desor) fishing tentacle and Chironex fleckeri Southcott nematocyst venoms

Toxinological and immunological studies of capillary electrophoresis fractionated Chrysaora quinquecirrha (Desor) fishing tentacle and Chironex fleckeri Southcott nematocyst venoms

Repeated runs of capillary electrophoresis (CE) were used to study partially-purified jellyfish nematocyst venom protein in concentrations sufficient to perform toxinological assays. Nematocyst venoms from Chironex fleckeri ( Cf) and Chysaora quinquecirrha were processed. The CE eluate was divided i...

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Bibliographic Details
Published in:Comparative biochemistry and physiology. Toxicology & pharmacology Vol. 128; no. 1; pp. 75 - 90
Main Authors: Bloom, D.A, Radwan, F.F.Y, Burnett, J.W
Format: Journal Article
Language:English
Published: United States Elsevier Inc 2001
Subjects:
Animals
Antibody Formation - drug effects
Capillary electrophoresis
Cells, Cultured
Chironex
Chironex fleckeri
Chrysaora
Chrysaora quinquecirrha
Cnidaria - physiology
Cnidarian Venoms - isolation & purification
Cnidarian Venoms - toxicity
Cytosensor
Electrophoresis, Capillary - methods
gel electrophoresis
Genes, T-Cell Receptor delta - drug effects
Hemolysin
Hemolysin Proteins - isolation & purification
Hemolysin Proteins - toxicity
Hemolysis - drug effects
Humans
Immunity, Cellular - drug effects
Jellyfish
Lethal factor
Liver - cytology
Liver - drug effects
Lymphocyte Activation - drug effects
Marine
Molecular Weight
Nematocysts
Sea Nettle, East Coast - physiology
T-Lymphocytes - drug effects
T-Lymphocytes - immunology
Toxins
Venoms
Chrysaora
Jellyfish
Nematocysts
Venoms
Capillary electrophoresis
Cytosensor
Toxins
Lethal factor
Hemolysin
Chironex
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Summary:Repeated runs of capillary electrophoresis (CE) were used to study partially-purified jellyfish nematocyst venom protein in concentrations sufficient to perform toxinological assays. Nematocyst venoms from Chironex fleckeri ( Cf) and Chysaora quinquecirrha were processed. The CE eluate was divided into quadrants by scanning protein content. The fourth fraction of both jellyfish venoms, contained proteins with the smallest molecular weight components, which were responsible for the highest hemolysins and the humoral and cell-mediated immunological activity. Cytotoxic Cf lethal factor activity against human liver cells was widely dispersed throughout both venoms but more prominent in fraction 4. A V β receptor human T-cell repertoire was not species-specific for either crude or fractionated jellyfish nematocyst venom.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:1532-0456
1878-1659
DOI:10.1016/S1532-0456(00)00180-0