Biochemical background of atherosclerotic heart lesion in an experiment

The authors investigated enzymic systems and corresponding substrate cycles that transport hydrogen across the mitochondrial membrane in a myocardium with experimental cholesterol-induced atherosclerosis. Sensitive spectrophotometric methods were used for assessing the activities of cytoplasmic and...

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Bibliographic Details
Published in:	Cor et vasa (English ed.) Vol. 19; no. 4-5; p. 355
Main Authors:	Gilmiyarova, F N, Sidorenkov, I V, Radomskaya, A M, Shpigel, A S
Format:	Journal Article
Language:	English
Published:	Czech Republic 1977
Subjects:	Animals Arteriosclerosis - enzymology Arteriosclerosis - metabolism Biological Transport Cytoplasm - enzymology Female L-Lactate Dehydrogenase - metabolism Malate Dehydrogenase - metabolism Male Mitochondria, Heart - enzymology NAD - metabolism Rabbits
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Summary:	The authors investigated enzymic systems and corresponding substrate cycles that transport hydrogen across the mitochondrial membrane in a myocardium with experimental cholesterol-induced atherosclerosis. Sensitive spectrophotometric methods were used for assessing the activities of cytoplasmic and mitochondrial enzymes: lactate and alpha-glycerphosphate dehydrogenases, and of characteristic mitochondrial enzymes: glutamate and beta-hydroxybutyrate dehydrogenases. Specific enzymological methods were used in determining the concentrations of lactic, pyruvic, glycerophosphoric, dihydroacetonephosphoric, malic, oxaloacetic, glutamic, alpha-ketoglutaric, acetoacetic, and beta-hydroxybutyric acids. The cytoplasmic NAD/NADH quotient was calculated. The investigators found a reduction of enzymic activities in the "shuttle" transport system studied, marked deviations from normal levels of their substrates, and a reduction of the NAD/NADH quotient by the factor 0.56. All these phenomena represent a biochemical background of a complex of symptoms characterizing severe myocardial lesion in experimental atherosclerosis.
ISSN:	0010-8650