Search Results - "Radlinska, M"

Cloning and characterization of the gene encoding a new DNA methyltransferase from Neisseria gonorrhoeae by Radlińska, M, Piekarowicz, A

“…A HindIII fragment of N. gonorrhoeae MS11 DNA coding for DNA methyltransferase (MTase) activity was cloned and expressed in E. coli AP1-200-9 cells. The…”
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Generating tandem repeats by cloning with double initiator fragments by Radlinska, M, Drabik, C E, Tong, W S, Lutter, L C

“…The ability to generate tandem repeats of a DNA sequence has proven important for a large variety of studies of DNA structure and function. The most commonly…”
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Structure prediction and phylogenetic analysis of a functionally diverse family of proteins homologous to the MT-A70 subunit of the human mRNA:m(6)A methyltransferase by Bujnicki, Janusz M, Feder, Marcin, Radlinska, Monika, Blumenthal, Robert M

“…MT-A70 is the S-adenosylmethionine-binding subunit of human mRNA:m(6)A methyl-transferase (MTase), an enzyme that sequence-specifically methylates adenines in…”
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Cloning of enterohemorrhagic Escherichia coli phage VT-2 dam methyltransferase by Radlinska, M, Bujnicki, J M

“…Enterobacterial GATC-specific DNA adenine methyltransferase (Dam) plays an essential role in regulation of DNA replication, methyl-directed mismatch repair,…”
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Molecular evolution of DNA-(cytosine-N4) methyltransferases: evidence for their polyphyletic origin by Bujnicki, J M, Radlinska, M

“…DNA N4-cytosine methyltransferases (N4mC MTases) are a family of S-adenosyl-L-methionine (AdoMet)-dependent MTases. Members of this family were previously…”
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Polyphyletic evolution of type II restriction enzymes revisited: two independent sources of second-hand folds revealed by Bujnicki, Janusz M, Rychlewski, Leszek, Radlinska, Monika

“…Using algorithms for protein sequence analysis we predict that some of the canonical type II and type IIS restriction enzymes have an active site with a…”
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Inference of relationships in the ‘twilight zone’ of homology using a combination of bioinformatics and site-directed mutagenesis: a case study of restriction endonucleases Bsp6I and PvuII by Pawlak, Sebastian D., Radlinska, Monika, Chmiel, Agnieszka A., Bujnicki, Janusz M., Skowronek, Krzysztof J.

“…Thus far, identification of functionally important residues in Type II restriction endonucleases (REases) has been difficult using conventional methods. Even…”
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Atomic model of the 5-methylcytosine-specific restriction enzyme McrA reveals an atypical zinc finger and structural similarity to beta beta alpha Me endonucleases by Bujnicki, MJ, Radlinska, M, Rychlewski, L

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Is the HemK family of putative S-adenosylmethionine-dependent methyltransferases a "missing" zeta subfamily of adenine methyltransferases? A hypothesis by Bujnicki, J M, Radlinska, M

“…Previous comparative studies revealed close similarity among various groups of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases (MTases),…”
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Topological Measurement of an A-tract Bend Angle: Comparison of the Bent and Straightened States by Tchernaenko, Vladimir, Radlinska, Monika, Drabik, Christopher, Bujnicki, Janusz, Halvorson, Herbert R, Lutter, Leonard C

“…It is well established that an A-tract imparts curvature to the DNA double helix. Constructs of such A-tracts have been used as bend standards in a large…”
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A theoretical model of restriction endonuclease NlaIV in complex with DNA, predicted by fold recognition and validated by site-directed mutagenesis and circular dichroism spectroscopy by Chmiel, Agnieszka A., Radlinska, Monika, Pawlak, Sebastian D., Krowarsch, Daniel, Bujnicki, Janusz M., Skowronek, Krzysztof J.

“…Restriction enzymes (REases) are commercial reagents commonly used in DNA manipulations and mapping. They are regarded as very attractive models for studying…”
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mRNA:guanine-N7 cap methyltransferases: identification of novel members of the family, evolutionary analysis, homology modeling, and analysis of sequence-structure-function relationships by Bujnicki, J M, Feder, M, Radlinska, M, Rychlewski, L

“…The 5'-terminal cap structure plays an important role in many aspects of mRNA metabolism. Capping enzymes encoded by viruses and pathogenic fungi are…”
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Molecular evolution of DNA-(cytosine-N4) methyItransferases: evidence for their polyphyletic origin by Bujnicki, Janusz M., Radlinska, Monika

“…DNA N4-cytosine methyltransferases (N4mC MTases) are a family of S-adenosyl-L-methionine (AdoMet)-dependent MTases. Members of this family were previously…”
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Structural characterization of two tandemly arranged DNA methyltransferase genes from Neisseria gonorrhoeae MS11: N4-cytosine specific M.NgoMXV and nonfunctional 5-cytosine-type M.NgoMorf2P by Radlinska, Monika, Bujnicki, Janusz M., Piekarowicz, Andrzej

“…Two adjacent genes encoding DNA methyltransferases (MTases) of Neisseria gonorrhoeae MS11, an active N4‐cytosine specific M.NgoMXV and an inactive 5‐cytosine…”
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Atomic model of the 5-methylcytosine-specific restriction enzyme McrA reveals an atypical zinc finger and structural similarity to betabetaalphaMe endonucleases by Bujnicki, J M, Radlinska, M, Rychlewski, L

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Restriction and modification systems of neisseria gonorrhoeae by Stein, Daniel C., Gunn, John S., Radlinska, Monika, Piekarowicz, Andrzej

“…An individual strain of Neisseria gonorrhoeae may produce up to 16 different DNA methytransferases (MTases). We have used a novel cloning system that is able…”
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Structure Prediction and Phylogenetic Analysis of a Functionally Diverse Family of Proteins Homologous to the MT-A70 Subunit of the Human mRNA:m6A Methyltransferase by Bujnicki, Janusz M., Feder, Marcin, Radlinska, Monika, Blumenthal, Robert M.

“…MT-A70 is the S-adenosylmethionine-binding subunit of human mRNA:m6A methyl-transferase (MTase), an enzyme that sequence-specifically methylates adenines in…”
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Cloning of the Haemophilus influenzae Dam methyltransferase and analysis of its relationship to the Dam methyltransferase encoded by the HP1 phage by Bujnicki, J M, Radlinska, M, Zaleski, P, Piekarowicz, A

“…In this paper we report cloning and experimental characterization of the DNA adenine methyltransferase (dam) gene from Haemophilus influenzae and comparison of…”
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Sensitivity of the restriction endonucleases HaeIII, BsrI, EaeI and CfrI to cytosine N4-methylation by Piekarowicz, A, Radlińska, M

“…HaeIII, BsrI and NgoII are isochizomers that recognize the sequence GGCC while EaeI and CfrI recognize the overlapping sequence YGGCCR. It has previously been…”
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Novel procedure for the detection of 5-methylcytosine by Radlińska, M, Skowronek, K

“…Bisulfite converts non-methylated cytosine in DNA to uracil leaving 5-methylcytosine unaltered. In this communication, we present a new approach omitting the…”
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