Analysis of HLA-G 14 bp Insertion/Deletion Polymorphism and HLA-G, ILT2 and ILT4 Expression in Head and Neck Squamous Cell Carcinoma Patients

HLA-G is the checkpoint molecule involved in the suppression of the immune response. Increased expression of HLA-G and its ILTs receptors have been correlated with tumor progression in various cancer types. In head and neck squamous cell carcinoma (HNSCC) tumors, the effect of HLA-G, ILT2 and ILT4 e...

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Bibliographic Details
Published in:	Diseases Vol. 12; no. 2; p. 34
Main Authors:	Durmanova, Vladimira, Tedla, Miroslav, Rada, Dusan, Bandzuchova, Helena, Kuba, Daniel, Suchankova, Magda, Ocenasova, Agata, Bucova, Maria
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 01-02-2024
Subjects:	Cancer Cell receptors Development and progression Gene deletion Gene expression Genetic aspects Head & neck cancer Head and neck cancer Head and neck carcinoma head and neck squamous cell carcinoma Health aspects Histocompatibility antigen HLA Histocompatibility antigens HLA histocompatibility antigens HLA-G Human papillomavirus ILT receptor Immune response Immunoglobulins Larynx Lymphocytes Medical prognosis Metastases Metastasis Microenvironments mRNA expression Otolaryngology Physiological aspects Polymorphism real-time PCR Single nucleotide polymorphisms Squamous cell carcinoma Tumors Slovakia United States > US Germany ILT receptor real-time PCR HLA-G head and neck squamous cell carcinoma mRNA expression
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Summary:	HLA-G is the checkpoint molecule involved in the suppression of the immune response. Increased expression of HLA-G and its ILTs receptors have been correlated with tumor progression in various cancer types. In head and neck squamous cell carcinoma (HNSCC) tumors, the effect of HLA-G, ILT2 and ILT4 expression on cancer development has to be explained. The 34 HNSCC patients and 98 controls were genotyped for the HLA-G 14 bp ins/del polymorphism. In HNSCC lesions, HLA-G, ILT2 and ILT4 mRNA expression was analysed using real-time PCR. The association between HLA-G, ILT2 and ILT4 mRNA expression and clinical variables (age at onset, TNM staging system and p16 positivity) was also evaluated. No genetic association between the HLA-G 14 bp ins/del and HNSCC risk was detected ( > 0.05). However, in the non-metastatic HNSCC group, a significantly higher HLA-G mRNA expression was noted in tumors in the T4 stage compared to those in the T1 and T2 stages ( = 0.0289). ILT2 mRNA expression was significantly increased in non-metastatic vs. metastatic tumors ( = 0.0269). Furthermore, a significantly higher ILT4 mRNA expression was noted in tumors in the T1+T2 stage compared to those in the T3 stage ( = 0.0495). Our results suggest that the HLA-G molecule creates an immunological microenvironment involved in HNSCC development.
ISSN:	2079-9721 2079-9721
DOI:	10.3390/diseases12020034