Developing Novel Fabrication and Optimisation Strategies on Aggregation-Induced Emission Nanoprobe/Polyvinyl Alcohol Hydrogels for Bio-Applications

Developing Novel Fabrication and Optimisation Strategies on Aggregation-Induced Emission Nanoprobe/Polyvinyl Alcohol Hydrogels for Bio-Applications

The current study describes a new technology, effective for readily preparing a fluorescent (FL) nanoprobe-based on hyperbranched polymer (HB) and aggregation-induced emission (AIE) fluorogen with high brightness to ultimately develop FL hydrogels. We prepared the AIE nanoprobe using a microfluidic...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Vol. 27; no. 3; p. 1002
Main Authors: Tavakoli, Javad, Shrestha, Jesus, Bazaz, Sajad R, Rad, Maryam A, Warkiani, Majid E, Raston, Colin L, Tipper, Joanne L, Tang, Youhong
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 02-02-2022
MDPI
Subjects:
aggregation-induced emission
Borax
Case studies
Cell viability
Chemical reactions
Emission analysis
Fabrication
Fluorescence
Fluorescent Dyes - chemistry
fluorescent hydrogel
fluorescent nanoprobe
Hydrogels
Hydrogels - chemistry
Hydrogen bonds
hyperbranched polymers
Mechanical properties
Microfluidics
Nanoparticles - chemistry
New technology
Polymers
Polyvinyl alcohol
Polyvinyl Alcohol - chemistry
Shear stress
Spectrometry, Fluorescence
Thin films
vortex fluidic device
vortex fluidic device
aggregation-induced emission
fluorescent hydrogel
fluorescent nanoprobe
hyperbranched polymers
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Summary:The current study describes a new technology, effective for readily preparing a fluorescent (FL) nanoprobe-based on hyperbranched polymer (HB) and aggregation-induced emission (AIE) fluorogen with high brightness to ultimately develop FL hydrogels. We prepared the AIE nanoprobe using a microfluidic platform to mix hyperbranched polymers (HB, generations 2, 3, and 4) with AIE (TPE-2BA) under shear stress and different rotation speeds (0-5 K RPM) and explored the FL properties of the AIE nanoprobe. Our results reveal that the use of HB generation 4 exhibits 30-times higher FL intensity compared to the AIE alone and is significantly brighter and more stable compared to those that are prepared using HB generations 3 and 2. In contrast to traditional methods, which are expensive and time-consuming and involve polymerization and post-functionalization to develop FL hyperbranched molecules, our proposed method offers a one-step method to prepare an AIE-HB nanoprobe with excellent FL characteristics. We employed the nanoprobe to fabricate fluorescent injectable bioadhesive gel and a hydrogel microchip based on polyvinyl alcohol (PVA). The addition of borax (50 mM) to the PVA + AIE nanoprobe results in the development of an injectable bioadhesive fluorescent gel with the ability to control AIEgen release for 300 min. When borax concentration increases two times (100 mM), the adhesion stress is more than two times bigger (7.1 mN/mm ) compared to that of gel alone (3.4 mN/mm ). Excellent dimensional stability and cell viability of the fluorescent microchip, along with its enhanced mechanical properties, proposes its potential applications in mechanobiology and understanding the impact of microstructure in cell studies.
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ISSN:1420-3049
1420-3049
DOI:10.3390/molecules27031002