Molecular cloning of two fast myosin heavy chain cDNAs from chicken embryo skeletal muscle

Molecular cloning of two fast myosin heavy chain cDNAs from chicken embryo skeletal muscle

Recombinant DNA clones containing sequences for two different types of myosin heavy chain (HC) genes from chicken embryonic skeletal muscle were constructed and analyzed. Specificity of the clones for myosin HC was demonstrated by hybrid-arrested translation, by hybridization to a 7.0-kb mRNA, and b...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS Vol. 78; no. 5; pp. 2843 - 2847
Main Authors: Umeda, P.K, Sinha, A.M, Jakovcic, S, Merten, S, Hsu, H.J, Subramanian, K.N, Zak, R, Rabinowitz, M.R
Format: Journal Article
Language:English
Published: United States National Academy of Sciences of the United States of America 01-05-1981
National Acad Sciences
Subjects:
Amino Acid Sequence
Amino acids
Animals
Base Sequence
Chick Embryo
Cloning, Molecular
Complementary DNA
DNA Restriction Enzymes
DNA, Recombinant - metabolism
Gels
Genetic Code
Hybridity
Melting
Messenger RNA
Microscopy, Electron
Muscles - metabolism
Myosins - genetics
Nucleic Acid Hybridization
Plasmids
Protein Biosynthesis
RNA
RNA, Messenger - genetics
Skeletal muscle
Transcription, Genetic
Untranslated regions
Online Access:Get full text
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Summary:Recombinant DNA clones containing sequences for two different types of myosin heavy chain (HC) genes from chicken embryonic skeletal muscle were constructed and analyzed. Specificity of the clones for myosin HC was demonstrated by hybrid-arrested translation, by hybridization to a 7.0-kb mRNA, and by comparison of DNA sequences with known amino acid sequences of rabbit skeletal muscle myosin HC. Restriction enzyme and electron-microscopic heteroduplex analysis showed the presence of two distinct but homologous cDNA sequences. Hybrid melting curves indicated that both types of sequences represent fast myosin HC sequences.
Bibliography:8121879
L50
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.78.5.2843