A Simple Double-Spin Closed Method for Preparing Platelet-Rich Plasma

To describe and analyze a new protocol for the extraction of platelet-rich plasma (PRP) for use in clinical practice and compare this technique with methods that have been previously described in the medical literature. Sixteen blood samples from healthy volunteers were collected. PRP was prepared u...

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Bibliographic Details
Published in:	Curēus (Palo Alto, CA) Vol. 14; no. 1; p. e20899
Main Authors:	Machado, Edilson S, Soares, Fabiano P, Yamaguchi, Roberta S, Felipone, William K, Meves, Robert, Souza, Tais Amara C, Topolniak, Roberto, Caldas, José P, Abreu, Ernani V, Rabelo Neto, Luiz S, Pinchemel, Pedro Vinicius S, Bredemeier, Markus
Format:	Journal Article
Language:	English
Published:	United States Cureus 03-01-2022
Subjects:	Orthopedics Pain Management Physical Medicine & Rehabilitation growth factors regenerative medicine orthobiologics orthopedics double-spin platelet-rich plasma platelets
Online Access:	Get full text
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Summary:	To describe and analyze a new protocol for the extraction of platelet-rich plasma (PRP) for use in clinical practice and compare this technique with methods that have been previously described in the medical literature. Sixteen blood samples from healthy volunteers were collected. PRP was prepared using our new double-spin technique, consisting of successive centrifugation of blood samples with two different spins, without opening the container. Descriptive analysis of cell counts in baseline and PRP samples was undertaken. Comparison between cell and platelet count in baseline and PRP samples, as well as the statistical analysis, were done. The mean platelet concentration ratio was 3.47 (SD: 0.85; 95% CI: 3.01-3.92; range: 2.48-5.71). The baseline whole blood platelet count correlated positively to the PRP platelet count (rP = 0.56; 95% CI: 0.09-0.88; P = 0.023). The PRP was enriched for lymphocytes and monocytes but presented significantly lower counts of neutrophils and eosinophils in comparison to baseline. Results show a safe and easily reproducible method to obtain PRP for use in clinical daily practice.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2168-8184 2168-8184
DOI:	10.7759/cureus.20899