Search Results - "RUMSH, Lev D."

Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia) by Kublanov, Ilya V, Perevalova, Anna A, Slobodkina, Galina B, Lebedinsky, Aleksander V, Bidzhieva, Salima K, Kolganova, Tatyana V, Kaliberda, Elena N, Rumsh, Lev D, Haertlé, Thomas, Bonch-Osmolovskaya, Elizaveta A

“…Samples of water from the hot springs of Uzon Caldera with temperatures from 68 to 87°C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin,…”
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Activity modulation of the oligopeptidase B from Serratia proteamaculans by site-directed mutagenesis of amino acid residues surrounding catalytic triad histidine by Mikhailova, Anna G., Rakitina, Tatiana V., Timofeev, Vladimir I., Karlinsky, David M., Korzhenevskiy, Dmitry A., Agapova, Yulia К., Vlaskina, Anna V., Ovchinnikova, Marina V., Gorlenko, Valentina A., Rumsh, Lev D.

“…Oligopeptidase B (OpdB; EC 3.4.21.83) is a trypsin-like peptidase belonging to the family of serine prolyl oligopeptidases; two-domain structure of the enzyme…”
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Molecular dynamics complemented by site-directed mutagenesis reveals significant difference between the interdomain salt bridge networks stabilizing oligopeptidases B from bacteria and protozoa in their active conformations by Petrenko, Dmitry E., Mikhailova, Anna G., Timofeev, Vladimir I., Agapova, Yulia К., Karlinsky, David M., Komolov, Aleksandr S., Korzhenevskiy, Dmitry A., Vlaskina, Anna V., Rumsh, Lev D., Rakitina, Tatiana V.

“…Oligopeptidases B (OpdBs) are trypsin-like peptidases from protozoa and bacteria that belong to the prolyl oligopeptidase (POP) family. All POPs consist of…”
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Evolution of inhibitor-resistant natural mutant forms of HIV-1 protease probed by pre-steady state kinetic analysis by Zakharova, Maria Yu, Kuznetsova, Alexandra A., Kaliberda, Elena N., Dronina, Maria A., Kolesnikov, Alexander V., Kozyr, Arina V., Smirnov, Ivan V., Rumsh, Lev D., Fedorova, Olga S., Knorre, Dmitry G., Gabibov, Alexander G., Kuznetsov, Nikita A.

“…Pre-steady state kinetic analysis of mechanistic features of substrate binding and processing is crucial for insight into the evolution of inhibitor-resistant…”
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Mutations in the proteolytic domain of Escherichia coli protease Lon impair the ATPase activity of the enzyme by Starkova, Natalie N, Koroleva, Ekaterina P, Rumsh, Lev D, Ginodman, Lev M, Rotanova, Tatyana V

“…Conserved residues of the proteolytic domain of Escherichia coli protease Lon, putative members of the classic catalytic triad (H665, H667, D676, and D743)…”
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Cloning, sequencing, expression, and characterization of thermostability of oligopeptidase B from Serratia proteamaculans, a novel psychrophilic protease by Mikhailova, Anna G., Khairullin, Rafil F., Demidyuk, Ilya V., Kostrov, Sergey V., Grinberg, Natalia V., Burova, Tatiana V., Grinberg, Valery Ya, Rumsh, Lev D.

“…•The gene of Serratia proteamaculans oligopeptidase B was cloned and sequenced.•The corresponding enzyme (PSP) was produced in Escherichia coli cells.•PSP…”
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new methodological approach to estimation of IgA1 and IgA2 content in human serum using recombinant IgA1 protease from Neisseria meningitidis by Zhigis, Larisa S, Kotel’nikova, Olga V, Vikhrov, Alexander A, Zinchenko, Aleksey A, Serova, Oksana V, Zueva, Vera S, Razgulyaeva, Olga A, Gordeeva, Elena A, Melikhova, Tatiana D, Nokel, Elena A, Alliluev, Alexander P, Drozhzhina, Elena Yu, Rumsh, Lev D

“…OBJECTIVES: A new approach to estimation of IgA subclass levels and IgA1/IgA2 ratio using enzymatically active and inactive forms of Neisseria meningitidis…”
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The ways of realization of high specificity and efficiency of enteropeptidase by Mikhailova, Anna G, Likhareva, Viktoria V, Teich, Niels, Rumsh, Lev D

“…Comparative substrate analysis of full-length bovine enteropeptidase and trypsin, bovine and human enteropeptidase light chains was performed using model…”
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Gel-immobilized enzymes as promising biocatalysts: Results from Indo-Russian collaborative studies by Markvicheva, Elena A., Lozinsky, Vladimir I., Plieva, Fatima M., Kochetkov, Konstantin A., Rumsh, Lev D., Zubov, Vitali P., Maity, Jyotirmoy, Kumar, Rajesh, Parmar, Virinder S., Belokon, Yury N.

“…Chemo-enzymatic methods constitute a promising approach to obtain various biologically active compounds, including enantiomerically pure substances. Entrapment…”
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Autolysis of bovine enteropeptidase heavy chain: evidence of fragment 118–465 involvement in trypsinogen activation by Mikhailova, Anna G, Rumsh, Lev D

“…Variations in bovine enteropeptidase (EP) activity were shown to result from autolysis caused by the loss of calcium ions; the cleavage sites were determined…”
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The isolated proteolytic domain of Escherichia coli ATP-dependent protease Lon exhibits the peptidase activity by Rasulova, Fatima S, Dergousova, Natalia I, Starkova, Natalie N, Melnikov, Edward E, Rumsh, Lev D, Ginodman, Lev M, Rotanova, Tatyana V

“…Selective protein degradation is an energy-dependent process performed by high-molecular-weight proteases. The activity of proteolytic components of these…”
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Analysis of crystal structures of aspartic proteinases: On the role of amino acid residues adjacent to the catalytic site of pepsin-like enzymes by Andreeva, N. S.

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Analysis of crystal structures of aspartic proteinases: On the role of amino acid residues adjacent to the catalytic site of pepsin‐like enzymes by Andreeva, Natalia S., Rumsh, Lev D.

“…To elucidate the role of amino acid residues adjacent to the catalytic site of pepsin‐like enzymes, we analyzed and compared the crystal structures of these…”
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Enteropeptidase: Structure, Function, and Application in Biotechnology by Mikhailova, Anna G, Rumsh, Lev D

“…A preparative method for purification of enteropeptidase (enterokinase) (EC 3. 4. 21.9) is developed. A highly purified form of this enzyme is stabilized by…”
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Immobilized Enzymes and Cells in Poly(N-Vinyl Caprolactam)-Based Hydrogels: Preparation, Properties, and Applications in Biotechnology and Medicine by Markvicheva, Elena A, Kuptsova, Svetlana V, Mareeva, Tatyana Yu, Vikhrov, Alexander A, Dugina, Tamara N, Strukova, Svetlana M, Belokon, Yury N, Kochetkov, Konstantin A, Baranova, Ekaterine N, Zubov, Vitali P, Poncelet, Denis, Parmar, Virinder S, Kumar, Rajesh, Rumsh, Lev D

“…A one-step mild method for entrapping animal cells and enzymes in macroporous composite poly (N-vinyl caprolactam)-calcium alginate (PVCL-CaAlg) hydrogels is…”
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HIV-I protease. Cloning, expression, and purification by Dergousova, N I, Amerik AYu, Volynskaya, A M, Rumsh, L D

“…A new method for obtaining HIV-I protease was suggested. Fusion proteins composed of the N-terminal fragment of human gamma-interferon and HIV-I protease…”
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Synthetic model and bioactive peptides as potential substrates for enteropeptidase by Likhareva, Viktoria V., Mikhailova, Anna G., Vaskovsky, Boris V., Garanin, Sergey K., Rumsh, Lev D.

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Enteropeptidase : Structure, function, and application in biotechnology by MIKHAILOVA, Anna G, RUMSH, Lev D

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Immobilized enzymes and cells in poly(N-vinyl caprolactam)-based hydrogels : Preparation, properties, and applications in biotechnology and medicine by MARKVICHEVA, Elena A, KUPTSOVA, Svetlana V, PONCELET, Denis, PARMAR, Virinder S, KUMAR, Rajesh, RUMSH, Lev D, MAREEVA, Tatyana Yu, VIKHROV, Alexander A, DUGINA, Tamara N, STRUKOVA, Svetlana M, BELOKON, Yury N, KOCHETKOV, Konstantin A, BARANOVA, Ekaterine N, ZUBOV, Vitali P

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Studies on the Mechanisms of Action of Proteolytic Enzymes Using Heavy Oxygen Exchange by ANTONOV, Vladimir K., GINODMAN, Lev M., RUMSH, Lev D., KAPITANNIKOV, Yurii V., BARSHEVSKAYA, Tatyana N., YAVASHEV, Libert P., GUROVA, Aleksandra G., VOLKOVA, Ludmila I.

“…Transpeptidation reactions catalyzed by chymotrypsin, pepsin, leucine aminopeptidase and thermolysin have been studied in heavy oxygen water (H218O). The 18O…”
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