Quantitative evaluation of urinary porphyrins as a measure of kidney mercury content and mercury body burden during prolonged methylmercury exposure in rats

Quantitative evaluation of urinary porphyrins as a measure of kidney mercury content and mercury body burden during prolonged methylmercury exposure in rats

Changes in urinary porphyrin excretion patterns (porphyrin profiles) during prolonged mercury exposure are attributable to mercury accumulation in the kidney and to consequent effects of Hg2+ on renal porphyrin metabolism. In the present study, we evaluated the quantitative relationship of urinary p...

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Bibliographic Details
Published in:Toxicological sciences Vol. 61; no. 2; pp. 234 - 240
Main Authors: PINGREE, Stephanie D, LYNNE SIMMONDS, P, RUMMEL, Kevin T, WOODS, James S
Format: Journal Article
Language:English
Published: Cary, NC Oxford University Press 01-06-2001
Subjects:
Animals
Biological and medical sciences
Body Burden
Chelating Agents - therapeutic use
Chemical and industrial products toxicology. Toxic occupational diseases
Dose-Response Relationship, Drug
Kidney - metabolism
Linear Models
Male
Medical sciences
Mercury - metabolism
Mercury - urine
Mercury Poisoning - urine
Metals and various inorganic compounds
Methylmercury Compounds - poisoning
Porphyrins - urine
Rats
Rats, Inbred F344
Regression Analysis
Spectrometry, Fluorescence
Statistics as Topic
Time Factors
Tissue Distribution
Toxicology
Unithiol - administration & dosage
Unithiol - therapeutic use
Biological fluid
Intraperitoneal administration
Chemical analysis
Rat
Biological marker
Mercaptoacid
Kidney
Tissue
Mercury Organic compounds
Quantitative analysis
Urine
Rodentia
Elimination
Oral administration
Sulfonic acid
Heavy metal
Toxicokinetics
Vertebrata
Mammalia
Correlation analysis
Porphyrin
Animal
Distribution
Chelating agent
Kinetics
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Summary:Changes in urinary porphyrin excretion patterns (porphyrin profiles) during prolonged mercury exposure are attributable to mercury accumulation in the kidney and to consequent effects of Hg2+ on renal porphyrin metabolism. In the present study, we evaluated the quantitative relationship of urinary porphyrin concentrations to mobilizable renal mercury content, using the metal chelator 2,3-dimercapto-1-propanesulfonic acid (DMPS) to modulate kidney mercury levels. Rats exposed to methylmercury hydroxide (MMH) at 10 ppm in drinking water for 6 weeks were treated with up to 3 consecutive doses of DMPS (100mg/kg, ip) at 72-h intervals. Consistent with previous findings, the concentrations of pentacarboxyl- (5-) and copro- (4-) porphyrins and of an atypical porphyrin specific to mercury exposure, precoproporphyrin, were significantly elevated in urine of MMH-exposed rats, compared with that of rats exposed to distilled water (dH2O) for the same period. Consecutive DMPS treatments of MMH-exposed rats significantly decreased kidney concentrations of total, as well as Hg2+ and CH3Hg+ species, and promoted increased urinary mercury excretion. Concomitantly, DMPS treatment decreased both kidney and urinary porphyrin concentrations, consistent with depletion of renal mercury levels. Regression analyses demonstrated a high correlation (r approximately 0.9) between prechelation urinary porphyrins and postchelation urinary mercury levels and also between prechelation urinary porphyrins and prechelation kidney mercury concentrations. These findings demonstrate that urinary porphyrin concentrations relate quantitatively to DMPS-mobilizable mercury in the kidney and, therefore, serve as a biochemical measure of renal mercury content.
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ISSN:1096-6080
1096-0929
1096-0929
DOI:10.1093/toxsci/61.2.234