Induction of competence in nonencapsulated and encapsulated strains of Haemophilus influenzae

Induction of competence in nonencapsulated and encapsulated strains of Haemophilus influenzae

A simple procedure for induction of competence in nonencapsulated and encapsulated strains of Haemophilus influenzae is described, which consists of growing cells without shaking in brain-heart infusion broth under aerobic conditions. Competence emerged at the end of the exponential phase and reache...

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Bibliographic Details
Published in:Current microbiology Vol. 19; no. 4; pp. 241 - 245
Main Authors: GROMKOVA, R. C, ROWJI, P. B, KOORNHOF, H. J
Format: Journal Article
Language:English
Published: New York, NY Springer 01-10-1989
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Microbiology
Encapsulation
Culture medium
Perfecting
Aerobiosis
Genetic transformation
Genetic competence
Haemophilus influenzae
Strain
Genetic transfer
Pasteurellaceae
Microorganism capsule
DNA
Microorganism culture
Bacteria
Technique
Nucleic acid
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Summary:A simple procedure for induction of competence in nonencapsulated and encapsulated strains of Haemophilus influenzae is described, which consists of growing cells without shaking in brain-heart infusion broth under aerobic conditions. Competence emerged at the end of the exponential phase and reached a peak at the stationary phase. In H. influenzae Rd competence was maintained for at least 6 h at 37 degree C, whereas in two encapsulated clinical isolates of H. influenzae type b a decrease in competence was observed after 4 h. Competence was maintained for 24 h at 22 degree C and 4 degree C as well as by freezing the cells in 15% glycerol and storing them at -70 degree C. Transformation frequencies of three chromosomal markers - streptomycin, nalidixic acid, and erythromycin resistance - were 0.5% to 1% in H. influenzae Rd and about tenfold lower in the two encapsulated clinical isolates of H. influenzae type b.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0343-8651
1432-0991
DOI:10.1007/BF01570169