Search Results - "ROBINSON, Margaret S"

Forty Years of Clathrin‐coated Vesicles by Robinson, Margaret S.

“…Forty years ago, Barbara Pearse purified coated vesicles from pig brain and discovered the protein clathrin. This review highlights some of the key discoveries…”
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Adaptor protein complexes and disease at a glance by Sanger, Anneri, Hirst, Jennifer, Davies, Alexandra K, Robinson, Margaret S

“…Adaptor protein (AP) complexes are heterotetramers that select cargo for inclusion into transport vesicles. Five AP complexes (AP-1 to AP-5) have been…”
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Role of the AP-5 adaptor protein complex in late endosome-to-Golgi retrieval by Hirst, Jennifer, Itzhak, Daniel N, Antrobus, Robin, Borner, Georg H H, Robinson, Margaret S

“…The AP-5 adaptor protein complex is presumed to function in membrane traffic, but so far nothing is known about its pathway or its cargo. We have used…”
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Distinct and Overlapping Roles for AP-1 and GGAs Revealed by the “Knocksideways” System by Hirst, Jennifer, Borner, Georg H.H., Antrobus, Robin, Peden, Andrew A., Hodson, Nicola A., Sahlender, Daniela A., Robinson, Margaret S.

“…Although adaptor protein complex 1 (AP-1) and Golgi-localized, γ ear-containing, ADP-ribosylation factor-binding proteins (GGAs) are both adaptors for…”
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The Molecular Basis for the Endocytosis of Small R-SNAREs by the Clathrin Adaptor CALM by Miller, Sharon E., Sahlender, Daniela A., Graham, Stephen C., Höning, Stefan, Robinson, Margaret S., Peden, Andrew A., Owen, David J.

“…SNAREs provide a large part of the specificity and energy needed for membrane fusion and, to do so, must be localized to their correct membranes. Here, we show…”
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AP-4 vesicles contribute to spatial control of autophagy via RUSC-dependent peripheral delivery of ATG9A by Davies, Alexandra K., Itzhak, Daniel N., Edgar, James R., Archuleta, Tara L., Hirst, Jennifer, Jackson, Lauren P., Robinson, Margaret S., Borner, Georg H. H.

“…Adaptor protein 4 (AP-4) is an ancient membrane trafficking complex, whose function has largely remained elusive. In humans, AP-4 deficiency causes a severe…”
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Tetherin is an exosomal tether by Edgar, James R, Manna, Paul T, Nishimura, Shinichi, Banting, George, Robinson, Margaret S

“…Exosomes are extracellular vesicles that are released when endosomes fuse with the plasma membrane. They have been implicated in various functions in both…”
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Rapid Inactivation of Proteins by Rapamycin-Induced Rerouting to Mitochondria by Robinson, Margaret S., Sahlender, Daniela A., Foster, Samuel D.

“…We have developed a method for rapidly inactivating proteins with rapamycin-induced heterodimerization. Cells were stably transfected with siRNA-resistant,…”
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The fifth adaptor protein complex by Hirst, Jennifer, Barlow, Lael D, Francisco, Gabriel Casey, Sahlender, Daniela A, Seaman, Matthew N J, Dacks, Joel B, Robinson, Margaret S

“…Adaptor protein (AP) complexes sort cargo into vesicles for transport from one membrane compartment of the cell to another. Four distinct AP complexes have…”
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The WDR11 complex facilitates the tethering of AP-1-derived vesicles by Navarro Negredo, Paloma, Edgar, James R., Manna, Paul T., Antrobus, Robin, Robinson, Margaret S.

“…Vesicluar transport of proteins from endosomes to the trans -Golgi network (TGN) is an essential cellular pathway, but much of its machinery is still unknown…”
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Multivariate proteomic profiling identifies novel accessory proteins of coated vesicles by Borner, Georg H H, Antrobus, Robin, Hirst, Jennifer, Bhumbra, Gary S, Kozik, Patrycja, Jackson, Lauren P, Sahlender, Daniela A, Robinson, Margaret S

“…Despite recent advances in mass spectrometry, proteomic characterization of transport vesicles remains challenging. Here, we describe a multivariate proteomics…”
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Stable endocytic structures navigate the complex pellicle of apicomplexan parasites by Koreny, Ludek, Mercado-Saavedra, Brandon N., Klinger, Christen M., Barylyuk, Konstantin, Butterworth, Simon, Hirst, Jennifer, Rivera-Cuevas, Yolanda, Zaccai, Nathan R., Holzer, Victoria J. C., Klingl, Andreas, Dacks, Joel B., Carruthers, Vern B., Robinson, Margaret S., Gras, Simon, Waller, Ross F.

“…Apicomplexan parasites have immense impacts on humanity, but their basic cellular processes are often poorly understood. Where endocytosis occurs in these…”
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Change your Tplate, change your fate: plant CME and beyond by Zhang, Yi, Persson, Staffan, Hirst, Jennifer, Robinson, Margaret S., van Damme, Daniel, Sánchez-Rodríguez, Clara

“…•The octameric TPLATE complex (TPC) is essential for initiation of CME in plants.•The TPC works in concert with the AP-2 complex.•The TPC most likely evolved…”
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A human genome-wide screen for regulators of clathrin-coated vesicle formation reveals an unexpected role for the V-ATPase by Kozik, Patrycja, Hodson, Nicola A., Sahlender, Daniela A., Simecek, Nikol, Soromani, Christina, Wu, Jiahua, Collinson, Lucy M., Robinson, Margaret S.

“…Clathrin-mediated endocytosis is essential for a wide range of cellular functions. We used a multi-step siRNA-based screening strategy to identify regulators…”
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Interaction between AP-5 and the hereditary spastic paraplegia proteins SPG11 and SPG15 by Hirst, Jennifer, Borner, Georg H H, Edgar, James, Hein, Marco Y, Mann, Matthias, Buchholz, Frank, Antrobus, Robin, Robinson, Margaret S

“…The AP-5 complex is a recently identified but evolutionarily ancient member of the family of heterotetrameric adaptor proteins (AP complexes). It is associated…”
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Loss of AP-5 results in accumulation of aberrant endolysosomes: defining a new type of lysosomal storage disease by Hirst, Jennifer, Edgar, James R, Esteves, Typhaine, Darios, Frédéric, Madeo, Marianna, Chang, Jaerak, Roda, Ricardo H, Dürr, Alexandra, Anheim, Mathieu, Gellera, Cinzia, Li, Jun, Züchner, Stephan, Mariotti, Caterina, Stevanin, Giovanni, Blackstone, Craig, Kruer, Michael C, Robinson, Margaret S

“…Adaptor proteins (AP 1-5) are heterotetrameric complexes that facilitate specialized cargo sorting in vesicular-mediated trafficking. Mutations in AP5Z1,…”
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Contribution of the clathrin adaptor AP-1 subunit µ1 to acidic cluster protein sorting by Navarro Negredo, Paloma, Edgar, James R, Wrobel, Antoni G, Zaccai, Nathan R, Antrobus, Robin, Owen, David J, Robinson, Margaret S

“…Acidic clusters act as sorting signals for packaging cargo into clathrin-coated vesicles (CCVs), and also facilitate down-regulation of MHC-I by HIV-1 Nef. To…”
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Characterization of TSET, an ancient and widespread membrane trafficking complex by Hirst, Jennifer, Schlacht, Alexander, Norcott, John P, Traynor, David, Bloomfield, Gareth, Antrobus, Robin, Kay, Robert R, Dacks, Joel B, Robinson, Margaret S

“…The heterotetrameric AP and F-COPI complexes help to define the cellular map of modern eukaryotes. To search for related machinery, we developed a…”
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Clathrin-Mediated Endocytosis in AP-2-Depleted Cells by Motley, Alison, Bright, Nicholas A., Matthew N. J. Seaman, Robinson, Margaret S.

“…We have used RNA interference to knock down the AP-2 μ2 subunit and clathrin heavy chain to undetectable levels in HeLaM cells. Clathrin-coated pits associated…”
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Fractionation profiling: a fast and versatile approach for mapping vesicle proteomes and protein-protein interactions by Borner, Georg H H, Hein, Marco Y, Hirst, Jennifer, Edgar, James R, Mann, Matthias, Robinson, Margaret S

“…We developed "fractionation profiling," a method for rapid proteomic analysis of membrane vesicles and protein particles. The approach combines quantitative…”
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