Search Results - "RIEF, Matthias"

Mechanically switching single-molecule fluorescence of GFP by unfolding and refolding by Ganim, Ziad, Rief, Matthias

“…Green fluorescent protein (GFP) variants are widely used as genetically encoded fluorescent fusion tags, and there is an increasing interest in engineering…”
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Single-molecule dissection of stacking forces in DNA by Kilchherr, Fabian, Wachauf, Christian, Pelz, Benjamin, Rief, Matthias, Zacharias, Martin, Dietz, Hendrik

“…We directly measured at the single-molecule level the forces and lifetimes of DNA base-pair stacking interactions for all stack sequence combinations. Our…”
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Calcium-dependent folding of single calmodulin molecules by Stigler, Johannes, Rief, Matthias

“…Calmodulin is the primary calcium binding protein in living cells. Its function and structure depend strongly on calcium concentration. We used single molecule…”
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Folding and assembly of the large molecular machine Hsp90 studied in single-molecule experiments by Jahn, Markus, Buchner, Johannes, Hugel, Thorsten, Rief, Matthias

“…Folding of small proteins often occurs in a two-state manner and is well understood both experimentally and theoretically. However, many proteins are much…”
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Extracellular rigidity sensing by talin isoform-specific mechanical linkages by Austen, Katharina, Ringer, Pia, Mehlich, Alexander, Chrostek-Grashoff, Anna, Kluger, Carleen, Klingner, Christoph, Sabass, Benedikt, Zent, Roy, Rief, Matthias, Grashoff, Carsten

“…The ability of cells to adhere and sense differences in tissue stiffness is crucial for organ development and function. The central mechanisms by which…”
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Patient preferences for development in MRI scanner design: a survey of claustrophobic patients in a randomized study by Iwan, Elisa, Yang, Jinhua, Enders, Judith, Napp, Adriane Elisabeth, Rief, Matthias, Dewey, Marc

“…Objective To investigate which magnetic resonance imaging (MRI) scanner designs claustrophobic patients prefer. Material/methods We analyzed questionnaires…”
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Ligand-Dependent Equilibrium Fluctuations of Single Calmodulin Molecules by Junker, Jan Philipp, Ziegler, Fabian, Rief, Matthias

“…Single-molecule force spectroscopy allows superb mechanical control of protein conformation. We used a custom-built low-drift atomic force microscope to…”
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The Complex Folding Network of Single Calmodulin Molecules by Stigler, Johannes, Ziegler, Fabian, Gieseke, Anja, Gebhardt, J. Christof M., Rief, Matthias

“…Direct observation of the detailed conformational fluctuations of a single protein molecule en route to its folded state has so far been realized only in…”
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Dynamic force sensing of filamin revealed in single-molecule experiments by Rognoni, Lorenz, Stigler, Johannes, Pelz, Benjamin, Ylänne, Jari, Rief, Matthias

“…Mechanical forces are important signals for cell response and development, but detailed molecular mechanisms of force sensing are largely unexplored. The…”
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Bioorthogonal protein-DNA conjugation methods for force spectroscopy by Synakewicz, Marie, Bauer, Daniela, Rief, Matthias, Itzhaki, Laura S.

“…Accurate and stable site-specific attachment of DNA molecules to proteins is a requirement for many single-molecule force spectroscopy techniques. The most…”
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Anisotropic Deformation Response of Single Protein Molecules by Dietz, Hendrik, Berkemeier, Felix, Bertz, Morten, Rief, Matthias

“…Single-molecule methods have given experimental access to the mechanical properties of single protein molecules. So far, access has been limited to mostly one…”
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Subnanometre enzyme mechanics probed by single-molecule force spectroscopy by Pelz, Benjamin, Žoldák, Gabriel, Zeller, Fabian, Zacharias, Martin, Rief, Matthias

“…Enzymes are molecular machines that bind substrates specifically, provide an adequate chemical environment for catalysis and exchange products rapidly, to…”
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Single-molecule force spectroscopy of toehold-mediated strand displacement by Walbrun, Andreas, Wang, Tianhe, Matthies, Michael, Šulc, Petr, Simmel, Friedrich C., Rief, Matthias

“…Toehold-mediated strand displacement (TMSD) is extensively utilized in dynamic DNA nanotechnology and for a wide range of DNA or RNA-based reaction circuits…”
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Exploring the Energy Landscape of GFP by Single-Molecule Mechanical Experiments by Dietz, Hendrik, Rief, Matthias, Lorimer, George H.

“…We use single-molecule force spectroscopy to drive single GFP molecules from the native state through their complex energy landscape into the completely…”
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charged linker of the molecular chaperone Hsp90 modulates domain contacts and biological function by Jahn, Markus, Rehn, Alexandra, Pelz, Benjamin, Hellenkamp, Björn, Richter, Klaus, Rief, Matthias, Buchner, Johannes, Hugel, Thorsten

“…Significance Many proteins consist of domains that are connected by flexible linkers. Because of their flexibility they are usually unresolved in crystal…”
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Elastic bond network model for protein unfolding mechanics by Dietz, Hendrik, Rief, Matthias

“…Recent advances in single molecule mechanics have made it possible to investigate the mechanical anisotropy of protein stability in great detail. A…”
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Ligand-Induced Changes of the Apparent Transition-State Position in Mechanical Protein Unfolding by Stigler, Johannes, Rief, Matthias

“…Force-spectroscopic measurements of ligand-receptor systems and the unfolding/folding of nucleic acids or proteins reveal information on the underlying energy…”
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Rigid DNA Beams for High-Resolution Single-Molecule Mechanics by Pfitzner, Emanuel, Wachauf, Christian, Kilchherr, Fabian, Pelz, Benjamin, Shih, William M., Rief, Matthias, Dietz, Hendrik

“…Bridging the gap: Rigid DNA linkers (blue, see picture) between microspheres (green) for high‐resolution single‐molecule mechanical experiments were…”
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Forced protein unfolding leads to highly elastic and tough protein hydrogels by Fang, Jie, Mehlich, Alexander, Koga, Nobuyasu, Huang, Jiqing, Koga, Rie, Gao, Xiaoye, Hu, Chunguang, Jin, Chi, Rief, Matthias, Kast, Juergen, Baker, David, Li, Hongbin

“…Protein-based hydrogels usually do not exhibit high stretchability or toughness, significantly limiting the scope of their potential biomedical applications…”
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titin-telethonin complex is a directed, superstable molecular bond in the muscle Z-disk by Bertz, Morten, Wilmanns, Matthias, Rief, Matthias

“…Mechanical stability of bonds and protein interactions has recently become accessible through single molecule mechanical experiments. So far, mechanical…”
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