A rheo-optical method for the study of aggregated particles : application to the characterization of ABO blood group monoclonal antibodies

A rheo-optical method for the study of aggregated particles : application to the characterization of ABO blood group monoclonal antibodies

The adhesive energy of monoclonal antibody induced agglutination can be assessed from the shear dissociation of agglutinates. Anti-A, anti-B, and anti-AB monoclonal antibodies agglutinate erythrocytes with variable strength, depending on the affinity of antibodies and on the antigenic density on the...

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Bibliographic Details
Published in:Rheologica acta Vol. 39; no. 6; pp. 595 - 600
Main Authors: STOLTZ, Jean-Francois, RASIA, Rodolphos Jose, VALVERDE, Juana Rosa, PLA, L, SONTAG, Danielle, RAPAILLE, Alain, MULLER, S
Format: Journal Article
Language:English
Published: Berlin Springer 01-11-2000
Springer Nature B.V
Subjects:
Adhesion tests
Agglutination
Antigens
Backscattering
Biological and medical sciences
Blood groups
Erythrocytes
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immunoglobulins
Immunohematology
Luminous intensity
Monoclonal antibodies
Parameters
Red blood cell immunology
Shear stress
Blood typing
Rheology
Backscattering
ABO(H)-System
Hemagglutination test
Red blood cell
Exploration
Monoclonal antibody
Adhesion
Blood group
Adhesive strength
Shear stress
Optical observation
Agglutination
Technique
Computer aid
Computer aided analysis
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Summary:The adhesive energy of monoclonal antibody induced agglutination can be assessed from the shear dissociation of agglutinates. Anti-A, anti-B, and anti-AB monoclonal antibodies agglutinate erythrocytes with variable strength, depending on the affinity of antibodies and on the antigenic density on the cell surface. The time required for an applied shear stress to dissociate cell agglutinates completely is depending on the strength of the agglutination reaction. The dissociation process under constant shear stress can be followed by measuring intensity of light backscattered by suspended particles. The obtained dissociation curves could be adjusted by an exponential expression governed by a parameter called characteristic time of dissociation. This parameter decreases with increasing antibody concentration. Integration of dissociation curves provided an approach of adhesive energy induced by monoclonal antibody.
ISSN:0035-4511
1435-1528
DOI:10.1007/s003970000109