Search Results - "RACHER, A. J"

Comparative proteomic analysis of GS-NSO murine myeloma cell lines with varying recombinant monoclonal antibody production rate by SMALES, C. M, DINNIS, D. M, STANSFIELD, S. H, ALETE, D, SAGE, E. A, BIRCH, J. R, RACHER, A. J, MARSHALL, C. T, JAMES, D. C

“…We have employed an inverse engineering strategy based on quantitative proteome analysis to identify changes in intracellular protein abundance that correlate…”
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On the Optimal Ratio of Heavy to Light Chain Genes for Efficient Recombinant Antibody Production by CHO Cells by Schlatter, Stefan, Stansfield, Scott H., Dinnis, Diane M., Racher, Andrew J., Birch, John R., James, David C.

“…Monoclonal antibodies (Mab) are heterotetramers consisting of an equimolar ratio of heavy chain (HC) and light chain (LC) polypeptides. Accordingly, most…”
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Effect of Viscosity upon Hydrodynamically Controlled Natural Aggregates of Animal Cells Grown in Stirred Vessels by Moreira, José L., Santana, Paula C., Feliciano, Ana S., Cruz, Pedro E., Racher, Andrew J., Griffiths, J. Bryan, Carrondo, Manuel J. T.

“…The effect of medium viscosity upon cell growth and aggregate characteristics of baby hamster kidney (BHK) cells cultivated in stirred tanks was evaluated. Two…”
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Expression of recombinant antibody and secreted alkaline phosphatase in mammalian cells. Influence of cell line and culture system upon production kinetics by RACHER, A. J, MOREIRA, J. L, ALVES, P. M, WIRTH, M, WEIDLE, U. H, HAUSER, H, CARRONDO, M. J. T, GRIFFITHS, J. B

“…The growth and productivity of an Sp2/0 cell line, F3b10, expressing a recombinant antibody (rAb) and BHK21 cells expressing either the same rAb from the same…”
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Cultural and physiological factors affecting expression of recombinant proteins by GRIFFITHS, J. B, RACHER, A. J

“…The variability in expression of recombinant proteins has been analyzed with regard to (a) comparison of clones from the same transfection experiment; (b)…”
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Studies on monoclonal antibody production by a hybridoma cell line (C1E3) immobilised in a fixed bed, porosphere culture system by Racher, A J, Looby, D, Griffiths, J B

“…The aim of this study was to investigate the potential of fixed beds of macroporous glass spheres as a production process for animal cell products. The growth,…”
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Use of lactate dehydrogenase release to assess changes in culture viability by Racher, A J, Looby, D, Griffiths, J B

“…This study reports the use of lactate dehydrogenase release to monitor changes in culture viability in flask culture and fixed bed, porosphere bioreactor…”
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Influence of ammonium ion and glucose on mAb production in suspension and fixed bed hybridoma cultures by Racher, A J, Looby, D, Griffiths, J B

“…Previous work indicated that mAb production by a mouse-mouse hybridoma, grown in a fixed bed of macroporous glass beads with a variable feed glutamine…”
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Investigation of parameters affecting a fixed bed bioreactor process for recombinant cell lines by RACHER, A. J, GRIFFITHS, J. B

“…A BHK 21 cell line expressing a recombinant antibody was grown in a fixed bed reactor (FBR) system using a porous support made of Siran glass beads. The…”
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Maximisation of perfusion systems and process comparison with batch-type cultures. Maximisation of perfusion cultures by Griffiths, J B, Looby, D, Racher, A J

“…A comparison of cell yields and monoclonal antibody productivity from the same hybridoma has been made in a wide range of cell bioreactors including both batch…”
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Culture of 293 cells in different culture systems : cell growth and recombinant adenovirus production by RACHER, A. J, FOOKS, A. R, GRIFFITHS, J. B

“…Growth of 293 cells (human embryonic kidney) was compared in various cell culture systems including static flasks, cell aggregates and a variety of porous…”
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Control of Culture Environment for Improved Polyethylenimine-Mediated Transient Production of Recombinant Monoclonal Antibodies by CHO Cells by Galbraith, Douglas J., Tait, Andrew S., Racher, Andrew J., Birch, John R., James, David C.

“…In this study we describe optimization of polyethylenimine (PEI)‐mediated transient production of recombinant protein by CHO cells by facile manipulation of a…”
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Comparative proteomic analysis of GS-NS0 murine myeloma cell lines with varying recombinant monoclonal antibody production rate by Smales, C.M., Dinnis, D.M., Stansfield, S.H., Alete, D., Sage, E.A., Birch, J.R., Racher, A.J., Marshall, C.T., James, D.C.

“…We have employed an inverse engineering strategy based on quantitative proteome analysis to identify changes in intracellular protein abundance that correlate…”
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Proteomic analysis of enriched microsomal fractions from GS-NS0 murine myeloma cells with varying secreted recombinant monoclonal antibody productivities by Alete, Daniel E., Racher, Andrew J., Birch, John R., Stansfield, Scott H., James, David C., Smales, C. Mark

“…The folding, transport and modification of recombinant proteins in the constitutive secretory pathway of eukaryotic cell expression systems are reported to be…”
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Evaluation of individual protein errors in silver-stained two-dimensional gels by Mark Smales, C, Birch, John R, Racher, Andrew J, Marshall, Carol T, James, David C

“…The relationship between spot volume and variation for all protein spots observed on large format 2D gels when utilising silver stain technology and a model…”
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Maximisation of perfusion systems and process comparison with batch-type cultures by Griffiths, J B, Looby, D, Racher, A J

“…A comparison of cell yields and monoclonal antibody productivity from the same hybridoma has been made in a wide range of cell bioreactors including both batch…”
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Maximisation of perfusion systems and process comparison with batch-type cultures. Maximisation of perfusion cultures: Cell culture engineering. III by GRIFFITHS, J. B, LOOBY, D, RACHER, A. J

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